Symbiotic performance of supernodulating soybean (glycine-max (L) merrill) mutants during development on different nitrogen regimes

Hansen, Alexander P., Peoples, Mark B., Gresshoff, Peter M., Atkins, Craig A., Pate, John S. and Carroll, Bernard J. (1989) Symbiotic performance of supernodulating soybean (glycine-max (L) merrill) mutants during development on different nitrogen regimes. Journal of Experimental Botany, 40 7: 715-724. doi:10.1093/jxb/40.7.715


Author Hansen, Alexander P.
Peoples, Mark B.
Gresshoff, Peter M.
Atkins, Craig A.
Pate, John S.
Carroll, Bernard J.
Title Symbiotic performance of supernodulating soybean (glycine-max (L) merrill) mutants during development on different nitrogen regimes
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 0022-0957
1460-2431
Publication date 1989-07-01
Sub-type Article (original research)
DOI 10.1093/jxb/40.7.715
Volume 40
Issue 7
Start page 715
End page 724
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
Growth and symbiotic performance of soybean (Glycine max (L.) Merrill) cv. Bragg and three of its induced nodulation mutants (nod49, non-nodulating; ntsl 116, intermediate supernodulator; nts1007, extreme supernodulator) were compared throughout development under different nitrogen regimes (0, 2, 5 and 10 mol nitrate m−3). Nitrogen fixation was assessed using 15N-isotope dilution and xylem sap analysis for ureide content. Both techniques confirmed a complete lack of N2 fixation activity in nod49. Plant reliance on nitrogen fixation by the other genotypes was dependent on the nitrate regime and the developmental stage. The ntsl007 and ntsl 116 mutants fixed more nitrogen than the parent cultivar in the presence of 10 mol m−3 nitrate in the nutrient solution, but higher input of symbiotically derived nitrogen was still insufficient to offset the amount of nitrogen removed in the harvested seed. However, the mutants utilized less nitrate for growth than Bragg. Comparison of estimates of N2 fixation derived from the 15N-dilution technique with those based on relative ureide content of xylem sap indicated that the latter offered a simple and reliable procedure for evaluating the symbiotic performance of supernodulating plants.
Keyword 15N-isotope dilution
Supernodulation
Ureides
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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