Use of the maize transposons activator and dissociation to show that phosphinothricin and spectinomycin resistance genes act non-cell-autonomously in tobacco and tomato seedlings

Jones, Jonathan D. G., Jones, David A., Bishop, Gerard J., Harrison, Kate, Carroll, Bernard J. and Scofield, Steven R. (1993) Use of the maize transposons activator and dissociation to show that phosphinothricin and spectinomycin resistance genes act non-cell-autonomously in tobacco and tomato seedlings. Transgenic Research, 2 2: 63-78. doi:10.1007/BF01969379


Author Jones, Jonathan D. G.
Jones, David A.
Bishop, Gerard J.
Harrison, Kate
Carroll, Bernard J.
Scofield, Steven R.
Title Use of the maize transposons activator and dissociation to show that phosphinothricin and spectinomycin resistance genes act non-cell-autonomously in tobacco and tomato seedlings
Journal name Transgenic Research   Check publisher's open access policy
ISSN 0962-8819
1573-9368
Publication date 1993-03
Sub-type Article (original research)
DOI 10.1007/BF01969379
Volume 2
Issue 2
Start page 63
End page 78
Total pages 16
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Abstract Cell-autonomous genes have been used to monitor the excision of both endogenous transposons in maize and Antirrhinum, and transposons introduced into transgenic plants. In tobacco and Arabidopsis, the streptomycin phosphotransferase (SPT) gene reveals somatic excision of the maize transposon Activator (Ac) as green sectors on a white background in cotyledons of seedlings germinated in the presence of streptomycin. Cotyledons of tomato seedlings germinated on streptomycin-containing medium do not bleach, suggesting that a different assay for transposon excision in tomato is desirable. We have tested the use of the spectinomycin resistance (SPEC) gene (aadA) and a Basta resistance (BAR) gene (phosphinothricin acetyltransferase, or PAT) for monitoring somatic excision of Ac in tobacco and tomato. Both genetic and molecular studies demonstrate that genotypically variegated individuals that carry clones of cells from which Ac or Ds have excised from either SPEC or BAR genes, can be phenotypically completely resistant to the corresponding antibiotic. This demonstrates that these genes act non-cell-autonomously, in contrast to the SPT gene in tobacco. Possible reasons for this difference are discussed.
Keyword Transposon
Activator
Dissociation
Cell-autonomous
Spectinomycin
Tomato
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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