Analysis of the chromosomal distribution of transposon-carrying T-Dnas in tomato using the inverse polymerase chain-reaction

Thomas, Colwyn M., Jones, David A., English, James J., Carroll, Bernard J., Bennetzen, Jeffrey L., Harrison, Kate, Burbidge, Alan, Bishop, Gerard J. and Jones, Jonathan D. G. (1994) Analysis of the chromosomal distribution of transposon-carrying T-Dnas in tomato using the inverse polymerase chain-reaction. Molecular and General Genetics, 242 5: 573-585.


Author Thomas, Colwyn M.
Jones, David A.
English, James J.
Carroll, Bernard J.
Bennetzen, Jeffrey L.
Harrison, Kate
Burbidge, Alan
Bishop, Gerard J.
Jones, Jonathan D. G.
Title Analysis of the chromosomal distribution of transposon-carrying T-Dnas in tomato using the inverse polymerase chain-reaction
Journal name Molecular and General Genetics
Publication date 1994-03
Sub-type Article
DOI 10.1007/BF00285281
Volume number 242
Issue number 5
ISSN 0026-8925;1432-1874
Start page 573
End page 585
Total pages 13
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract We are developing a system for isolating tomato genes by transposon mutagenesis. In maize and tobacco, the transposon Activator (Ac) transposes preferentially to genetically linked sites. To identify transposons linked to various target genes, we have determined the RFLP map locations of Ac- and Dissociation (Ds)-carrying T-DNAs in a number of transformants. T-DNA flanking sequences were isolated using the inverse polymerase chain reaction (IPCR) and located on the RFLP map of tomato. The authenticity of IPCR reaction products was tested by several criteria including nested primer amplification, DNA sequence analysis and PCR amplification of the corresponding insertion target sequences. We report the RFLP map locations of 37 transposon-carrying T-DNAs. We also report the map locations of nine transposed Ds elements. T-DNAs were identified on all chromosomes except chromosome 6. Our data revealed no apparent chromosomal preference for T-DNA integration events. Lines carrying transposons at known map locations have been established which should prove a useful resource for isolating tomato genes by transposon mutagenesis.
Keyword Tomato
Agrobacterium T-Dna
Inverse Pcr
Activator
Transposon tagging
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collection: School of Chemistry and Molecular Biosciences
 
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