Germinal transpositions of the maize element dissociation from T-Dna loci in tomato

Carroll, B.J., Klimyuk, V.I., Thomas, C.M., Bishop, G.J., Harrison, K. and Jones, J.D.G. (1995) Germinal transpositions of the maize element dissociation from T-Dna loci in tomato. Genetics, 139 1: 407-420.

Author Carroll, B.J.
Klimyuk, V.I.
Thomas, C.M.
Bishop, G.J.
Harrison, K.
Jones, J.D.G.
Title Germinal transpositions of the maize element dissociation from T-Dna loci in tomato
Journal name Genetics   Check publisher's open access policy
ISSN 0016-6731
Publication date 1995-01
Sub-type Article (original research)
Volume 139
Issue 1
Start page 407
End page 420
Total pages 14
Place of publication Bethesda, MD, United States
Publisher Genetics Society of America
Language eng
Abstract We have analyzed the pattern of germinal transpositions of artificial Dissociation (Ds) transposons in tomato. T-DNA constructs carrying Ds were transformed into tomato, and the elements were trans-activated by crossing to lines transformed with a stabilized Activator (sAc) that expressed the transposase gene. The sAc T-DNA carried a GUS gene to monitor its segregation. The Ds elements were inserted in a marker gene so that excision from the T-DNA could be monitored. The Ds elements also carried a genetic marker that was intended to be used for reinsertion selection of the elements after excision. Unfortunately, this gene was irreversibly inactivated on crossing to sAc. Germinal excision frequencies of Ds averaged 15-40%, but there was large variation between and within plants. Southern hybridization analysis of stable transposed Ds elements indicated that although unique transpositions predominate, early transposition events can lead to large clonal sectors in the germline of developing plants and to sibling offspring carrying the same transposition event. Multiple germinal transpositions from three different loci were examined for uniqueness, and 15 different transpositions were identified from each of three T-DNA loci that carried a single independent Ds. These were mapped relative to the donor T-DNA loci, and for each locus 70-80% of the transposed elements were closely linked to the donor site.
Keyword Transposable element
Transgenic tomato
Ac transposition
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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