Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch

Olsen, Peter B., Schembri, Mark A., Gally, David L. and Klemm, Per (1998) Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch. FEMS Microbiology Letters, 162 1: 17-23. doi:10.1111/j.1574-6968.1998.tb12973.x


Author Olsen, Peter B.
Schembri, Mark A.
Gally, David L.
Klemm, Per
Title Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch
Journal name FEMS Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
1574-6968
Publication date 1998-05
Sub-type Article (original research)
DOI 10.1111/j.1574-6968.1998.tb12973.x
Volume 162
Issue 1
Start page 17
End page 23
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract Phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a 314-bp DNA element. This DNA switch directs transcription of fimA, encoding the major type 1 fimbrial subunit, in the on orientation but not in the off orientation. Inversion of the DNA element requires either FimB (confers both on and off orientations) or FimE (confers off orientation). Here we show, by chromosomally located fimB- and fimE-lacZ cassettes in isogenic strain sets differing only in the hns locus, how the global regulator H-NS affects the expression of type 1 fimbriae. H-NS was found to downregulate fimB and time in a temperature-dependent manner which affected the genes inversely at 30°C and 37°C. By gel-retardation assays H- NS binding was demonstrated to the regions containing the fimB promoter and the time promoter, respectively. In vitro recombination analysis suggested no direct involvement of H-NS in the inversion of the phase switch. Rather than directly affecting the switching process per se, it appeared that the orientation of this element was affected by the differential temperature modulation of H-NS of the fimB and time genes. Taken together the results suggest that H-NS modulates expression of type 1 fimbriae in a way which seems to favor a fimbriate state at the mammalian body temperature.
Keyword H-NS
H-NS binding
Temperature modulation of gene expression
Type 1 fimbriae
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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