Bioaccumulation of heavy metals by fimbrial designer adhesins

Schembri, Mark A., Kjaergaard, Kristian and Klemm, Per (1999) Bioaccumulation of heavy metals by fimbrial designer adhesins. FEMS Microbiology Letters, 170 2: 363-371. doi:10.1111/j.1574-6968.1999.tb13396.x

Author Schembri, Mark A.
Kjaergaard, Kristian
Klemm, Per
Title Bioaccumulation of heavy metals by fimbrial designer adhesins
Journal name FEMS Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
Publication date 1999-01
Sub-type Article (original research)
DOI 10.1111/j.1574-6968.1999.tb13396.x
Volume 170
Issue 2
Start page 363
End page 371
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Formatted abstract
Naturally occurring adhesins bind to specific molecular targets in a lock-and-key fashion due to the composition of the binding domain of the adhesin. By introduction of random peptide libraries in a suitable surface exposed carrier protein it is possible to create and select designer adhesins with novel binding affinities. Type 1 fimbriae are surface organelles of Escherichia coli which mediate d-mannose sensitive binding to different host surfaces through the FimH adhesin, an integral part of these organelles. We have studied the ability of the FimH adhesin to display random peptide sequences. By serial selection and enrichment procedures specific sequences were identified which conferred the ability on recombinant cells to adhere to various metal oxides (PbO2, CoO, MnO2, Cr2O3). The properties inherent in these sequences permitted the distinct recognition of metals to varying degrees, indicating that this system allows for the isolation of peptide sequences with a variety of binding avidities. These studies demonstrate the potential and versatility of the FimH display system for presenting random peptide sequences. In addition, the possibility exists for the construction of microorganisms for the bioaccumulation of heavy metals from the environment.
Keyword Bioremediation
Designer adhesin
Fimbrial peptide display
Heavy metal binder
Type 1 fimbria
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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