Antigen 43 and type 1 fimbriae determine colony morphology of Escherichia coli K-12

Hasman, Henrik, Schembri, Mark A. and Klemm, Per (2000) Antigen 43 and type 1 fimbriae determine colony morphology of Escherichia coli K-12. Journal of Bacteriology, 182 4: 1089-1095. doi:10.1128/JB.182.4.1089-1095.2000

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Author Hasman, Henrik
Schembri, Mark A.
Klemm, Per
Title Antigen 43 and type 1 fimbriae determine colony morphology of Escherichia coli K-12
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
Publication date 2000-02
Sub-type Article (original research)
DOI 10.1128/JB.182.4.1089-1095.2000
Open Access Status File (Publisher version)
Volume 182
Issue 4
Start page 1089
End page 1095
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Colony morphology has been used as an important identification and characterization criterion in bacteriology for many decades. However, the molecular mechanisms underlying the appearance of different colony types have been given little attention. The synthesis of O antigen is defunct in Escherichia coli K-12, and colonies should accordingly only appear to be rough. However, previous reports have noted the presence of different interchangeable colony morphology types. In this study we have addressed the influence of two phase-variable surface structures, antigen 43 and type 1 fimbriae, on colony morphology. Due to differential expression of these structures, four different colony phenotypes could be distinguished. By creating and studying defined mutants of the respective loci, i.e., flu and fim, we conclude that the presence or absence of the corresponding gene products on the cells correlates with the observed colony morphology forms. Interestingly, the habitat specificity of bacteria under static liquid conditions seems to correlate with the colony phenotypes.
Keyword Colony morphology
Escherichia coli K-12
Colony morphology types
Antigen 43
Type 1 fimbriae
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 60 times in Thomson Reuters Web of Science Article | Citations
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