Wolbachia uses host microRNAs to manipulate host gene expression and facilitate colonization of the dengue vector Aedes aegypti

Hussain, Mazhar, Frentiu, Francesca D., Moreira, Luciano A., O'Neill, Scott L. and Asgari, Sassan (2011) Wolbachia uses host microRNAs to manipulate host gene expression and facilitate colonization of the dengue vector Aedes aegypti. Proceedings of the National Academy of Science of the United States of America - PNAS, 108 22: 9250-0255. doi:10.1073/pnas.1105469108


Author Hussain, Mazhar
Frentiu, Francesca D.
Moreira, Luciano A.
O'Neill, Scott L.
Asgari, Sassan
Title Wolbachia uses host microRNAs to manipulate host gene expression and facilitate colonization of the dengue vector Aedes aegypti
Formatted title
Wolbachia uses host microRNAs to manipulate host gene expression and facilitate colonization of the dengue vector Aedes aegypti
Journal name Proceedings of the National Academy of Science of the United States of America - PNAS   Check publisher's open access policy
ISSN 0027-8424
1091-6490
Publication date 2011-05-31
Sub-type Article (original research)
DOI 10.1073/pnas.1105469108
Open Access Status Not Open Access
Volume 108
Issue 22
Start page 9250
End page 0255
Total pages 6
Place of publication Washington, DC, United States
Publisher National Academy of Sciences
Collection year 2012
Language eng
Formatted abstract
The obligate endosymbiont Wolbachia pipientis is found in a wide
range of invertebrates where they are best known for manipulating
host reproduction. Recent studies have shown that Wolbachia
also can modulate the lifespan of host insects and interfere with
the development of human pathogens in mosquito vectors. Despite
considerable study, very little is known about the molecular
interactions between Wolbachia and its hosts that might mediate
these effects. Using microarrays, we show that the microRNA
(miRNA) profile of the mosquito, Aedes aegypti, is significantly
altered by the wMelPop-CLA strain of W. pipientis. We found that
a host miRNA (aae-miR-2940) is induced after Wolbachia infection
in both mosquitoes and cell lines. One target of aae-miR-2940 is
the Ae. aegypti metalloprotease gene. Interestingly, expression of
the target gene was induced after Wolbachia infection, ectopic
expression of the miRNA independent of Wolbachia, or transfection
of an artificial mimic of the miRNA into mosquito cells. We
also confirmed the interaction of aae-miR-2940 with the target
sequences using GFP as a reporter gene. Silencing of the metalloprotease
gene in both Wolbachia-infected cells and adult mosquitoes
led to a significant reduction in Wolbachia density, as did
inhibition of the miRNA in cells. These results indicate that manipulation
of the mosquito metalloprotease gene via aae-miR-2940 is
crucial for efficient maintenance of the endosymbiont. This report
shows how Wolbachia alters the host miRNA profile and provides
insight into the mechanisms of host manipulation used by this
widespread endosymbiont.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biological Sciences Publications
 
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Created: Thu, 23 Jun 2011, 20:27:39 EST by Miss Francesca Frentiu on behalf of School of Biological Sciences