Comparison of the second internal transcribed spacer (Ribosomal DNA) from populations and species of fasciolidae (Digenea)

Adlard, Robert D., Barker, Stephen C., Blair, David and Cribb, Thomas H. (1993) Comparison of the second internal transcribed spacer (Ribosomal DNA) from populations and species of fasciolidae (Digenea). International Journal for Parasitology, 23 3: 423-425. doi:10.1016/0020-7519(93)90022-Q


Author Adlard, Robert D.
Barker, Stephen C.
Blair, David
Cribb, Thomas H.
Title Comparison of the second internal transcribed spacer (Ribosomal DNA) from populations and species of fasciolidae (Digenea)
Journal name International Journal for Parasitology   Check publisher's open access policy
ISSN 0020-7519
1879-0135
Publication date 1993-05
Sub-type Article (original research)
DOI 10.1016/0020-7519(93)90022-Q
Volume 23
Issue 3
Start page 423
End page 425
Total pages 3
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
The nucleotide sequence of the 3' end of the second internal transcribed spacer region (ITS2) was determined for four isolates of Fasciola hepatica, two isolates of Fasciola gigantica, one isolate of Fascioloides magna and one isolate of Fasciola sp. from Japan. Intraspecific sequence divergence was negligible. Sequence divergence between F. hepatica and F. gigantica was 2.8% and between Fasciola hepatica and Fascioloides magna was 13.2%. The sequence of Fasciola sp. from Japan matched closely that of F. gigantica. This study demonstrates variability in nucleotide sequence within the ITS2 region which allows discrimination between species of Fasciolidae.
Keyword Fasciola-hepatica
Fasciola-gigantica
Fascioloides-magna
rDNA
Ribosomal DNA
Internal transcribed spacer
ITS
PCR
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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