Widespread expression of human alpha 1-antitrypsin in transgenic mice revealed by in situ hybridization

Koopman, Peter, Povey, Sue and Lovell-Badge, Robin H. (1989) Widespread expression of human alpha 1-antitrypsin in transgenic mice revealed by in situ hybridization. Genes and Development, 3 1: 16-25. doi:10.1101/gad.3.1.16


Author Koopman, Peter
Povey, Sue
Lovell-Badge, Robin H.
Title Widespread expression of human alpha 1-antitrypsin in transgenic mice revealed by in situ hybridization
Journal name Genes and Development   Check publisher's open access policy
ISSN 0890-9369
1549-5477
Publication date 1989-01
Sub-type Article (original research)
DOI 10.1101/gad.3.1.16
Open Access Status DOI
Volume 3
Issue 1
Start page 16
End page 25
Total pages 10
Place of publication Woodbury, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Abstract In situ hybridization is a powerful means of identifying sites of gene expression. We used this technique to examine the spatial and developmental control of transcription of the human alpha 1-antitrypsin (alpha 1 AT) gene in transgenic mice carrying this gene and extensive 5'- and 3'-flanking sequences. In addition to expression in yolk sac and liver, human alpha 1AT RNA was detected in gut, stomach, pancreas, nasal epithelium, pharynx, bronchi, spinal ganglia, and ossifying cartilage of transgenic fetuses at 14.5 days post coitum (dpc). In transgenic adults, expression was no longer found in the pancreas but was found in the kidney and salivary gland. In each tissue, expression was confined to a specific cell population. This pattern of alpha 1AT expression was found to correlate with that seen in several fetal and adult human tissues. These results suggest a wider role of alpha 1AT in human physiology and development than previously suspected, and they demonstrated the potential value of this approach in delineating the physiological role of human proteins. Expression of the endogenous alpha 1AT gene in mice was confined to a limited, but overlapping, set of tissues, suggesting that the cis-acting DNA sequences that regulate the expression of the human and mouse genes interact differently with transcription factors present in mouse cells.
Keyword a1-Antitrypsin
Transgenic mice
In situ hybridization
Tissue-specific expression
Developmental regulation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 54 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Thu, 23 Jun 2011, 13:54:42 EST by System User on behalf of Institute for Molecular Bioscience