SOX9 binds DNA, activates transcription, and coexpresses with type II collagen during chondrogenesis in the mouse

Ng, Ling-Jim, Wheatley, Susan, Muscat, George E. O., Conway-Campbell, John, Bowles, Jo, Wright, Edwina, Bell, Donald M., Cheah, Kathryn S. E. and Koopman, Peter (1997) SOX9 binds DNA, activates transcription, and coexpresses with type II collagen during chondrogenesis in the mouse. Developmental Biology, 183 1: 108-121. doi:10.1006/dbio.1996.8487


Author Ng, Ling-Jim
Wheatley, Susan
Muscat, George E. O.
Conway-Campbell, John
Bowles, Jo
Wright, Edwina
Bell, Donald M.
Cheah, Kathryn S. E.
Koopman, Peter
Title SOX9 binds DNA, activates transcription, and coexpresses with type II collagen during chondrogenesis in the mouse
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
1095-564X
Publication date 1997-03
Sub-type Article (original research)
DOI 10.1006/dbio.1996.8487
Volume 183
Issue 1
Start page 108
End page 121
Total pages 14
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Abstract Two lines of evidence suggest that the Sry-related gene Sox9 is important for chondrogenesis in mammalian embryos. Sox9 mRNA is expressed in chondrogenic condensations in mice, and mutations in human SOX9 are known to cause skeletal dysplasia. We show here that mouse SOX9 protein is able to bind to a SOX/SRY consensus motif in DNA and contains a modular transcriptional activation domain, consistent with a role for SOX9 as a transcription factor acting on genes involved in cartilage development. One such gene is Col2a1, which encodes type II collagen, the major structural component of cartilage. We have compared, in detail, the expression of Sox9 and Col2a1 during mouse development. In chondrogenic tissues the expression profiles of the two genes were remarkably similar. Coexpression was detected in some nonchondrogenic tissues such as the notochord, otic vesicle, and neural tube, but others such as heart and lung differed in their expression of the two genes. Immunohistochemistry using an antibody specific for SOX9 revealed that expression of SOX9 protein mirrored the distribution of Sox9 mRNA. Our results suggest that SOX9 protein is involved in the regulation of Col2a1 during chondrogenesis, but that this regulation is likely to depend on additional cofactors.
Keyword Autosomal sex reversal
Sry-related gene
Limb cartilage differentiation
Campomelic dysplasia
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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