Expansion of the Ago gene family in the teleost clade

McFarlane, Lindsey, Svingen, Terje, Braasch, Ingo, Koopman, Peter, Schartl, Manfred and Wilhelm, Dagmar (2011) Expansion of the Ago gene family in the teleost clade. Development Genes and Evolution, 221 2: 95-104. doi:10.1007/s00427-011-0363-7

Author McFarlane, Lindsey
Svingen, Terje
Braasch, Ingo
Koopman, Peter
Schartl, Manfred
Wilhelm, Dagmar
Title Expansion of the Ago gene family in the teleost clade
Formatted title
Expansion of the Ago gene family in the teleost clade
Journal name Development Genes and Evolution   Check publisher's open access policy
ISSN 0949-944X
Publication date 2011-06
Sub-type Article (original research)
DOI 10.1007/s00427-011-0363-7
Volume 221
Issue 2
Start page 95
End page 104
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2012
Language eng
Formatted abstract
AGO proteins are universal effectors of eukaryotic small RNA-directed regulatory pathways. In this study, we used a comparative genomics approach to explore the AGO sub-family in the teleost clade. We identified five Ago homologues in teleost genomes, one more than encoded in other vertebrate clades. The additional teleost homologue was preserved most likely due to the differential retention of regulatory elements following the fish-specific genome duplication event that occurred approximately 350 million years ago. Analysis of all five Ago genomic loci in teleosts revealed that orthologues contain specific, conserved sequence elements in non-coding regions indicating that the teleost Ago paralogues are differentially regulated. This was supported by qRT-PCR analysis that showed differential expression of the zebrafish homologues across development and between adult tissues indicating stage and tissue-specific function of individual AGO proteins. Multiple sequence alignments showed not only that all teleost homologues possess critical residues for AGO function, but also that teleost homologues contain multiple orthologue-specific features, indicative of structural diversification. Notably, these are retained throughout the vertebrate lineage arguing these may be important for orthologue-specific functions.
Keyword Argonaute
Non-coding RNA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
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