A factor produced by feeder cells which inhibits embryonal carcinoma cell differentiation. Characterization and partial purification

Koopman, Peter and Cotton, Richard G. H. (1984) A factor produced by feeder cells which inhibits embryonal carcinoma cell differentiation. Characterization and partial purification. Experimental Cell Research, 154 1: 233-242. doi:10.1016/0014-4827(84)90683-9


Author Koopman, Peter
Cotton, Richard G. H.
Title A factor produced by feeder cells which inhibits embryonal carcinoma cell differentiation. Characterization and partial purification
Journal name Experimental Cell Research   Check publisher's open access policy
ISSN 0014-4827
1090-2422
Publication date 1984-09
Sub-type Article (original research)
DOI 10.1016/0014-4827(84)90683-9
Volume 154
Issue 1
Start page 233
End page 242
Total pages 10
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Abstract Medium conditioned by STO mouse fibroblast cells inhibited both the spontaneous differentiation of NG2 embryonal carcinoma cells and the differentiation of F9 embryonal carcinoma cells induced by retinoic acid. This effect was due to a differentiation retarding factor (DRF). Reduction in DRF activity in conditioned medium by boiling and by pronase treatment suggested the involvement of a polypeptide, which had an apparent molecular weight of 57000 on gel filtration. A 28-fold purification of DRF was achieved. DRF delayed but did not prevent the extensive differentiation observed after prolonged culture of NG2 colonies. Conditioned medium could be successfully used to replace feeder cells in NG2 stock cultures. Media conditioned by a variety of other cell types also contained differentiation retarding activity.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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