Widespread expression of the testis-determining gene SRY in a marsupial

Harry, Jenny L., Koopman, Peter, Brennan, Francine E., Graves, Jennifer A. and Renfree, Marilyn B. (1995) Widespread expression of the testis-determining gene SRY in a marsupial. Nature Genetics, 11 3: 347-349. doi:10.1038/ng1195-347


Author Harry, Jenny L.
Koopman, Peter
Brennan, Francine E.
Graves, Jennifer A.
Renfree, Marilyn B.
Title Widespread expression of the testis-determining gene SRY in a marsupial
Journal name Nature Genetics   Check publisher's open access policy
ISSN 1061-4036
1546-1718
Publication date 1995-11
Sub-type Letter to editor, brief commentary or brief communication
DOI 10.1038/ng1195-347
Volume 11
Issue 3
Start page 347
End page 349
Total pages 3
Place of publication New York, NY, United States
Publisher Nature Publishing Group
Language eng
Abstract Total RNA was extracted from pooled tammar fetuses and neonatal gonads and analysed for SRY expression using reverse transcription and polymerase chain reaction (RT-PCR). In the tammar, the gonadal primordium is first apparent at day 21 of the 26.5 day gestation. By day 25 the cytoplasmic:nuclear ratio in male pre-Sertoli cells is greater than in female cells, but it is not until two days post-partum that seminiferous cords are obvious with putative Leydig cells appearing in the interstitium. We initially tested for the presence of SRY transcripts from the day after the genital ridge is established (day 22 of gestation) until two days after the development of seminiferous tubules (day 4 post partum). Surprisingly, SRY expression was detected throughout this entire developmental period in male fetal tissue and in gonads from male pouch young (Fig. 1a). We therefore extended our sampling period from day 19 of gestation (early head-fold; ~20 somites) to 40 days after birth and established that in the tammar SRY is expressed from at least 6 days before the appearance of Sertoli cells until long after the immature testis has fully differentiated (Fig. 1b). Thus, expression of the tammar SRY gene does not result in immediate Sertoli cell differentiation.
Keyword Sex-Determining Region
Y-Chromosome
Mouse Testis
Differentiation
Zfy
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Letter to editor, brief commentary or brief communication
Collection: Institute for Molecular Bioscience - Publications
 
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