A generalized transducing phage (ΦIF3) for the genomically sequenced Serratia marcescens strain Db11: A tool for functional genomics of an opportunistic human pathogen

Petty, Nicola K., Foulds, Ian J., Pradel, Elizabeth, Ewbank, Jonathan J. and Salmond, George P. C. (2006) A generalized transducing phage (ΦIF3) for the genomically sequenced Serratia marcescens strain Db11: A tool for functional genomics of an opportunistic human pathogen. Microbiology, 152 6: 1701-1708. doi:10.1099/mic.0.28712-0


Author Petty, Nicola K.
Foulds, Ian J.
Pradel, Elizabeth
Ewbank, Jonathan J.
Salmond, George P. C.
Title A generalized transducing phage (ΦIF3) for the genomically sequenced Serratia marcescens strain Db11: A tool for functional genomics of an opportunistic human pathogen
Formatted title
A generalized transducing phage (ΦIF3) for the genomically sequenced Serratia marcescens strain Db11: A tool for functional genomics of an opportunistic human pathogen
Journal name Microbiology   Check publisher's open access policy
ISSN 1350-0872
1465-2080
Publication date 2006-06
Sub-type Article (original research)
DOI 10.1099/mic.0.28712-0
Volume 152
Issue 6
Start page 1701
End page 1708
Total pages 8
Place of publication Reading, Berks., United Kingdom
Publisher Society for General Microbiology
Language eng
Formatted abstract
A bacteriophage (φIF3) capable of mediating generalized transduction in Serratia marcescens strain Db11 has been isolated and characterized. The genome of this Serratia strain has recently been sequenced and is likely to become the reference strain for S. marcescens researchers. φIF3 is most likely a virulent phage, which can transduce markers at frequencies of 10-6 transductants per p.f.u. It has a lipopolysaccharide receptor and was determined to have a latent period of 50 min and a burst size of approximately 100 phages. The phage DNA was resistant to digestion with restriction enzymes. Electron microscopy showed φIF3 to be a member of the family Myoviridae. This is the first report of a generalized transducing phage able to infect Db11 and this phage will be a valuable tool for functional genomic analysis of the pathogen host.
© 2006 SGM.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Wed, 22 Jun 2011, 20:03:25 EST by Dr Nicola Petty on behalf of School of Chemistry & Molecular Biosciences