Evidence for a lineage of virulent bacteriophages that target Campylobacter

Timms, Andrew R., Cambray-Young, Joanna, Scott, Andrew E., Petty, Nicola K., Connerton, Phillippa L., Clarke, Louise, Seeger, Kathy, Quail, Mike, Cummings, Nicola, Maskell, Duncan J., Thomson, Nicholas R. and Connerton, Ian F. (2010) Evidence for a lineage of virulent bacteriophages that target Campylobacter. BMC Genomics, 11 1: 214-1-214-10. doi:10.1186/1471-2164-11-214


Author Timms, Andrew R.
Cambray-Young, Joanna
Scott, Andrew E.
Petty, Nicola K.
Connerton, Phillippa L.
Clarke, Louise
Seeger, Kathy
Quail, Mike
Cummings, Nicola
Maskell, Duncan J.
Thomson, Nicholas R.
Connerton, Ian F.
Title Evidence for a lineage of virulent bacteriophages that target Campylobacter
Formatted title
Evidence for a lineage of virulent bacteriophages that target Campylobacter
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2010
Sub-type Article (original research)
DOI 10.1186/1471-2164-11-214
Open Access Status DOI
Volume 11
Issue 1
Start page 214-1
End page 214-10
Total pages 10
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Our understanding of the dynamics of genome stability versus gene flux within bacteriophage lineages is limited. Recently, there has been a renewed interest in the use of bacteriophages as 'therapeutic' agents; a prerequisite for their use in such therapies is a thorough understanding of their genetic complement, genome stability and their ecology to avoid the dissemination or mobilisation of phage or bacterial virulence and toxin genes. Campylobacter, a food-borne pathogen, is one of the organisms for which the use of bacteriophage is being considered to reduce human exposure to this organism.
Results: Sequencing and genome analysis was performed for two Campylobacter bacteriophages. The genomes were extremely similar at the nucleotide level (≥ 96%) with most differences accounted for by novel insertion sequences, DNA methylases and an approximately 10 kb contiguous region of metabolic genes that were dissimilar at the sequence level but similar in gene function between the two phages. Both bacteriophages contained a large number of radical S-adenosylmethionine (SAM) genes, presumably involved in boosting host metabolism during infection, as well as evidence that many genes had been acquired from a wide range of bacterial species. Further bacteriophages, from the UK Campylobacter typing set, were screened for the presence of bacteriophage structural genes, DNA methylases, mobile genetic elements and regulatory genes identified from the genome sequences. The results indicate that many of these bacteriophages are related, with 10 out of 15 showing some relationship to the sequenced genomes.
Conclusions: Two large virulent Campylobacter bacteriophages were found to show very high levels of sequence conservation despite separation in time and place of isolation. The bacteriophages show adaptations to their host and possess genes that may enhance Campylobacter metabolism, potentially advantaging both the bacteriophage and its host. Genetic conservation has been shown to extend to other Campylobacter bacteriophages, forming a highly conserved lineage of bacteriophages that predate upon campylobacters and indicating that highly adapted bacteriophage genomes can be stable over prolonged periods of time.
© 2010 Timms et al; licensee BioMed Central Ltd.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Article # 214

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Wed, 22 Jun 2011, 19:39:20 EST by Dr Nicola Petty on behalf of School of Chemistry & Molecular Biosciences