Triphasic pattern in the ex vivo response of human proliferative phase endometrium to oestrogens

Punyadeera, C., Marbaix, E., Kamps, R., Galant, C., Nap, A., de Goeij, A., Ederveen, A. and Groothuis, P. (2004) Triphasic pattern in the ex vivo response of human proliferative phase endometrium to oestrogens. Journal of Steroid Biochemistry and Molecular Biology, 92 3: 175-185. doi:10.1016/j.jsbmb.2004.06.005


Author Punyadeera, C.
Marbaix, E.
Kamps, R.
Galant, C.
Nap, A.
de Goeij, A.
Ederveen, A.
Groothuis, P.
Title Triphasic pattern in the ex vivo response of human proliferative phase endometrium to oestrogens
Journal name Journal of Steroid Biochemistry and Molecular Biology   Check publisher's open access policy
ISSN 0960-0760
1879-1220
Publication date 2004-10
Sub-type Article (original research)
DOI 10.1016/j.jsbmb.2004.06.005
Volume 92
Issue 3
Start page 175
End page 185
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Pergamon
Language eng
Abstract The aim of this study was to evaluate the ex vivo oestrogen responsiveness of human proliferative phase endometrium using short-term explant cultures. The effects of oestrogen (17β-E2) on proliferation and the expression of oestrogen-responsive genes known to be involved in regulating endometrial function were evaluated. Three distinct response patterns could be distinguished: (1) the menstrual (M) phase pattern (cycle days 2-5), which is characterised by a complete lack in the proliferative response to 17β-E2, while an increased expression of AR (2.6-fold, P < 0.01), PR (2.7-fold, P < 0.01) and COX-2 (3.5-fold, P < 0.01) at the mRNA level was observed and a similar upregulation was also found for AR, PR and COX-2 at the protein level; (2) the early proliferative (EP) phase pattern (cycle days 6-10) with 17β-E2 enhanced proliferation in the stroma (1.7-fold, P < 0.05), whereas the expression of AR, PR and COX-2 were not affected at the mRNA and protein levels and ER-α mRNA and protein levels were significantly reduced by 17β-E2; (3) the late proliferative (LP) phase pattern (cycle days 11-14), which is characterised by a moderate stimulation of proliferation (1.4-fold, P < 0.05) and PR mRNA expression (1.7-fold, P < 0.01) by 17β-E2. In conclusion, three distinct response patterns to 17β-E2 could be identified with respect to proliferation and the expression of known oestrogen-responsive genes in human proliferative phase endometrium explant cultures.
Keyword Steroid receptors
Steroid hormones
Maturation
Explant cultures
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Available online 28 October 2004.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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