Progesterone regulation of implantation-related genes: new insights into the role of oestrogen

Dassen, H., Punyadeera, C., Kamps, R., Klomp, J., Dunselman, G., Dijcks, F., de Goeij, A., Ederveen, A. and Groothuis, P. (2007) Progesterone regulation of implantation-related genes: new insights into the role of oestrogen. Cellular and Molecular Life Sciences, 64 7-8: 1009-1032. doi:10.1007/s00018-007-6553-9


Author Dassen, H.
Punyadeera, C.
Kamps, R.
Klomp, J.
Dunselman, G.
Dijcks, F.
de Goeij, A.
Ederveen, A.
Groothuis, P.
Title Progesterone regulation of implantation-related genes: new insights into the role of oestrogen
Journal name Cellular and Molecular Life Sciences   Check publisher's open access policy
ISSN 1420-682X
1420-9071
Publication date 2007-04
Sub-type Article (original research)
DOI 10.1007/s00018-007-6553-9
Volume 64
Issue 7-8
Start page 1009
End page 1032
Total pages 24
Place of publication Basel, Switzerland
Publisher Birkhaeuser Verlag AG
Language eng
Formatted abstract
Genomic profiling was performed on explants of late proliferative phase human endometrium after 24-h treatment with progesterone (P) or oestradiol and progesterone (17β-E2+P) and on explants of menstrual phase endometrium treated with 17β-E2+P. Gene expression was validated with real-time PCR in the samples used for the arrays, in endometrium collected from early and mid-secretory phase endometrium, and in additional experiments performed on new samples collected in the menstrual and late proliferative phase. The results show that late proliferative phase human endometrium is more responsive to progestins than menstrual phase endometrium, that the expression of several genes associated with embryo implantation (i.e. thrombomodulin, monoamine oxidase A, SPARC-like 1) can be induced by P in vitro, and that genes that are fully dependent on the continuous presence of 17β-E2 during P exposure can be distinguished from those that are P-dependent to a lesser extent. Therefore, 17β-E2 selectively primes implantation-related genes for the effects of P.
Keyword Human endometrium
Explant cultures
Global gene expression
Oestradiol
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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