Estrogen metabolizing enzymes in endometrium and endometriosis

Dassan, H., Punyadeera, C., Kamps, R., Delvoux, B., Van Langendonckt, A., Donnez, J., Husen, B., Thole, H., Dunselman, G. and Groothuis, P. (2007) Estrogen metabolizing enzymes in endometrium and endometriosis. Human Reproduction, 22 12: 3148-3158. doi:10.1093/humrep/dem310

Author Dassan, H.
Punyadeera, C.
Kamps, R.
Delvoux, B.
Van Langendonckt, A.
Donnez, J.
Husen, B.
Thole, H.
Dunselman, G.
Groothuis, P.
Title Estrogen metabolizing enzymes in endometrium and endometriosis
Journal name Human Reproduction   Check publisher's open access policy
ISSN 0268-1161
Publication date 2007-12
Sub-type Article (original research)
DOI 10.1093/humrep/dem310
Volume 22
Issue 12
Start page 3148
End page 3158
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
BACKGROUND: Estradiol (E2) is an important promoter of the growth of both eutopic and ectopic endometrium. The findings with regard to the expression and activity of steroidogenic enzymes in endometrium of controls, in endometrium of endometriosis patients and in endometriotic lesions are not consistent.

METHODS: In this study, we have looked at the mRNA expression and protein levels of a range of steroidogenic enzymes [aromatase, 17ß-hydroxysteroid dehydrogenases (17ß-HSD) type 1, 2 and 4, estrogen sulfotransferase (EST) and steroid sulfatase (STS)] in eutopic and ectopic endometrium of patients (n = 14) with deep-infiltrative endometriosis as well as in disease-free endometrium (n = 48) using real-time PCR and immunocytochemistry. In addition, we evaluated their menstrual cycle-related expression patterns, and investigated their steroid responsiveness in explant cultures.

RESULTS: Aromatase and 17ß-HSD type 1 mRNA levels were extremely low in normal human endometrium, while mRNAs for types 2 and 4 17ß-HSD, EST and STS were readily detectable. Only 17ß-HSD type 2 and EST genes showed sensitivity to progesterone in normal endometrium. Types 1 and 2 17ß-HSD and STS protein was detected in normal endometrium using new polyclonal antibodies.

CONCLUSIONS: In endometriosis lesions, the balance is tilted in favor of enzymes producing E2. This is due to a suppression of types 2 and 4 17ß-HSD, and an increased expression of aromatase and type 1 17ß-HSD in ectopic endometrium.
Keyword Endometriosis
Steroidogenic enzymes
Estrogen metabolism
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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