Haemoglobin expression in human endometrium

Kamps, R., Punyadeera, C., Dijcks, F., de Goeij, A., Ederveen, A., Dunselman, G. and Groothuis, P. (2008) Haemoglobin expression in human endometrium. Human Reproduction, 23 3: 635-641. doi:10.1093/humrep/dem430

Author Kamps, R.
Punyadeera, C.
Dijcks, F.
de Goeij, A.
Ederveen, A.
Dunselman, G.
Groothuis, P.
Title Haemoglobin expression in human endometrium
Journal name Human Reproduction   Check publisher's open access policy
ISSN 0268-1161
Publication date 2008-03
Sub-type Article (original research)
DOI 10.1093/humrep/dem430
Volume 23
Issue 3
Start page 635
End page 641
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
BACKGROUND: The general concept that haemoglobin is only a carrier protein for oxygen and carbon dioxide is challenged since recent studies have shown haemoglobin expression in non-erythroid cells and the protection of haemoglobin against oxidative and nitrosative stress. Using microarrays, we previously showed expression of haemoglobins α, ß, ð and γ and the haeme metabolizing enzyme, haeme oxygenase (HO)-1 in human endometrium.

Using real-time quantitative PCR, haemoglobin α, ß, ð and γ, and HO-1 mRNA levels were assessed throughout the menstrual cycle (n = 30 women). Haemoglobin and HO-1 protein levels in the human endometrium were assessed with immunohistochemistry. For steroid responsiveness, menstrual and late proliferative-phase endometrial explants were cultured for 24 h in the presence of vehicle (0.1% ethanol), estradiol (17ß-E2, 1 nM), progestin (Org 2058, 1 nM) or 17ß-E2+Org 2058 (1 nM each).

RESULTS: All haemoglobins and the HO-1 were expressed in normal human endometrium. Haemoglobin mRNA and protein expression did not vary significantly during the menstrual cycle. Explant culture with Org 2058 or 17ß-E2+Org 2058 increased haemoglobin γ mRNA expression (P < 0.05). HO-1 mRNA levels, and not protein levels, were significantly higher during the menstrual (M)-phase of the cycle (P < 0.05), and were down-regulated by Org 2058 in M-phase explants and by 17ß-E2+Org 2058 in LP-phase explants, versus control (P < 0.05).

CONCLUSIONS: The haemoglobin-HO-1 system may be required to ensure adequate
regulation of the bioavailability of haeme, iron and oxygen in human endometrium.
Keyword Haemoglobin
Haeme oxygenase
Human endometrium
Menstrual cycle
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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