Oral contraceptives prevent the development of endometriosis in the chicken chorioallantoic membrane model

Nap, Annemiek W., Groothuis, Patrick G., Punyadeera, Chamindie, Klein-Hitpass, Ludger, Kamps, Rik, Delvoux, Bert and Dunselman, Gerard A. J. (2008) Oral contraceptives prevent the development of endometriosis in the chicken chorioallantoic membrane model. Contraception, 78 3: 257-265. doi:10.1016/j.contraception.2008.05.003


Author Nap, Annemiek W.
Groothuis, Patrick G.
Punyadeera, Chamindie
Klein-Hitpass, Ludger
Kamps, Rik
Delvoux, Bert
Dunselman, Gerard A. J.
Title Oral contraceptives prevent the development of endometriosis in the chicken chorioallantoic membrane model
Journal name Contraception   Check publisher's open access policy
ISSN 0010-7824
1879-0518
Publication date 2008-09
Sub-type Article (original research)
DOI 10.1016/j.contraception.2008.05.003
Volume 78
Issue 3
Start page 257
End page 265
Total pages 9
Place of publication Philadelphia, United States
Publisher Elsevier
Language eng
Formatted abstract
Background

Fundamental and genetic differences between women in the endometrium may cause some to develop endometriosis, whereas others do not. Oral contraceptives (OC) may have an effect on the endometrium, rendering the development of endometriosis less likely.

Study Design


Endometrium from women using OC (OCE) and menstrual endometrium (ME) from normal cycling women were transplanted onto the chicken chorioallantoic membrane (CAM), and endometriosis-like lesion formation was evaluated. Microarray gene expression profiling was performed to identify differentially expressed genes in the endometrium from these groups. Microarray data were validated by real-time PCR.

Results


Less endometriosis-like lesions were formed after transplantation of OCE than after transplantation of ME (p<.05). Most of the differentially expressed genes belong to the TGFβ superfamily. Real-time PCR validation revealed that inhibin βA (INHBA) expression was significantly decreased in OCE as compared to ME.

Conclusion


OC use affects the characteristics of endometrium, rendering it less potent to develop into endometriosis.
Keyword Chorioallantoic membrane model
Endometrium
Endometriosis
Microarray analysis
Oral contraceptives
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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