Proliferation and differentiation in the human breast during pregnancy

Suzuki, R., Atherton, A. J., O'Hare, M. J., Entwistle, A., Lakhani, S. R. and Clarke, C. (2000) Proliferation and differentiation in the human breast during pregnancy. Differentiation, 66 2-3: 106-115. doi:10.1046/j.1432-0436.2000.660205.x

Author Suzuki, R.
Atherton, A. J.
O'Hare, M. J.
Entwistle, A.
Lakhani, S. R.
Clarke, C.
Title Proliferation and differentiation in the human breast during pregnancy
Journal name Differentiation   Check publisher's open access policy
ISSN 0301-4681
Publication date 2000-10
Sub-type Article (original research)
DOI 10.1046/j.1432-0436.2000.660205.x
Volume 66
Issue 2-3
Start page 106
End page 115
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
Using multiple immunofluorescence labelling on human breast tissues obtained and freshly frozen at the 12th, 15th, and 18th weeks of pregnancy, we have shown that markers of mammary functional differentiation, milk proteins (β-casein and κ-casein), are synthesised by actively cycling (Ki67 positive) as well as non-cycling (Ki67 negative) cells. These results demonstrate that functional differentiation/maturation does not coincide with loss of proliferative potential in human mammary luminal epithelial cells. In addition, we have examined expression patterns of integrin subunits (α1, α2, α3, α6, β1, and β4) and extracellular matrix components (laminin, fibronectin, collagen I, and collagen IV), since they have been shown to exert influences on mammary differentiation and morphogenesis in vitro. Compared to human breast tissues obtained from non-pregnant women, a decrease in α2 labelling on luminal epithelial cells was observed, particularly in expanding acini that showed abundant Ki67 positivity. The expression patterns of other integrin subunits, however, did not change, indicating that the expression patterns of most integrins existing prior to pregnancy are sufficient to support the morphological and functional development associated with milk protein synthesis.
Keyword Breast
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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