Bactericidal mechanisms of human breast milk leukocytes

Johnson, Dianne F., France, Gene L., Marmer, Daniel J. and Steele, Russell W. (1980) Bactericidal mechanisms of human breast milk leukocytes. Infection and Immunity, 28 2: 314-318.

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Author Johnson, Dianne F.
France, Gene L.
Marmer, Daniel J.
Steele, Russell W.
Title Bactericidal mechanisms of human breast milk leukocytes
Journal name Infection and Immunity
ISSN 1098-5522
1070-6313
Publication date 1980-05
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 28
Issue 2
Start page 314
End page 318
Total pages 5
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract The functional capacity of human breast milk phagocytes was evaluated with both bactericidal and biochemical assays. Acridine orange was used as a vital stain for bacteria to directly visualize phagocytosis and killing. Bactericidal capabilities were further examined by colony count and chemiluminescent methods. Cytocentrifuged specimens stained for myeloperoxidase exhibited enzyme activity in breast milk leukocytes equal to that of peripheral neutrophils. A radioisotopic assay of hexose monophosphate shunt activity demonstrated metabolic activity in breast milk leukocytes greater than that in peripheral blood neutrophils. However, the chemiluminescent response of breast cells was negligible, apparently the result of quenching secondary to fat present in the milk; preincubation of human blood leukocytes with the fatty layer of breast milk produced similar inhibition in the chemiluminescence assay. By most parameters, breast milk phagocytes are at least equal to blood neutrophils.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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