Increased platelet-derived growth factor production and intimal thickening during healing of Dacron grafts in a canine model

Margolin, D. A., Kaufman, B. R., Deluca, D. J., Fox, P. L. and Graham, L. M. (1993). Increased platelet-derived growth factor production and intimal thickening during healing of Dacron grafts in a canine model. In: Annual Meeting of the Midwestern Vascular Surgical Society, Cleveland Ohio, USA, (858-867). 11-12 September 1992.

Author Margolin, D. A.
Kaufman, B. R.
Deluca, D. J.
Fox, P. L.
Graham, L. M.
Title of paper Increased platelet-derived growth factor production and intimal thickening during healing of Dacron grafts in a canine model
Conference name Annual Meeting of the Midwestern Vascular Surgical Society
Conference location Cleveland Ohio, USA
Conference dates 11-12 September 1992
Journal name Journal of Vascular Surgery   Check publisher's open access policy
Place of Publication Philadelphia, PA, United States
Publisher Mosby
Publication Year 1993
Sub-type Fully published paper
ISSN 0741-5214
1097-6809
Volume 17
Issue 5
Start page 858
End page 867
Total pages 10
Language eng
Formatted Abstract/Summary
Purpose: Growth factor production by endothelial cells on grafts may play a role in the development of intimal hyperplasia and subsequent graft failure.

Methods: To study the relationship between platelet-derived growth factor production and graft healing, 26 beagles underwent placement of 20 cm long, 6 mm internal diameter, knitted Dacron thoracoabdominal grafts, either seeded with autologous endothelial cells (n = 14) or unseeded controls (n = 12). The grafts and adjacent arteries were removed 4 or 20 weeks after implantation for measurement of platelet-derived growth factor production in organ culture, endothelial cell coverage, and intimal thickness.

Results: Midgraft platelet-derived growth factor production by seeded graft segments increased from 41 ± 6 to 148 ± 27 pg/cm2/72 hr (p < 0.002) between 4 and 20 weeks. This was accompanied by a significant increase in inner-capsule thickness. Platelet-derived growth factor production by control graft segments also increased from 58 ± 21 to 163 ± 42 pg (p < 0.05) and was similar to that of seeded grafts despite more rapid endothelialization of seeded grafts. The increase in growth factor production by Dacron grafts was greater than that of the expanded polytetrafluoroethylene grafts studied previously despite similar endothelial cell coverage.

Conclusion: This increase corresponded with the rapid appearance of smooth muscle cells in the pseudointima of Dacron grafts, suggesting that these cells may be responsible for the observed increase in platelet-derived growth factor production.
Keyword Smooth-Muscle Cells
Organ-Culture System
Factor Like Mitogen
Cellular Proliferation
Ptfe Prostheses
Vascular Grafts
Arterial Injury
Mechanisms
Expression
Lesions
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Conference Paper
Collection: School of Medicine Publications
 
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