Differential monocytic cell adherence to specific anatomic regions of the canine aorta

Margolina, David A., Madura, James A., de la Motte, Carol A., Fox, Paul L., Dicorleto, Paul E. and Graham, Linda M (1995) Differential monocytic cell adherence to specific anatomic regions of the canine aorta. Journal of Vascular Research, 32 4: 266-274. doi:10.1159/000159101


Author Margolina, David A.
Madura, James A.
de la Motte, Carol A.
Fox, Paul L.
Dicorleto, Paul E.
Graham, Linda M
Title Differential monocytic cell adherence to specific anatomic regions of the canine aorta
Journal name Journal of Vascular Research   Check publisher's open access policy
ISSN 1018-1172
1423-0135
Publication date 1995-07
Sub-type Article (original research)
DOI 10.1159/000159101
Volume 32
Issue 4
Start page 266
End page 274
Total pages 9
Place of publication Basel, Switzerland
Publisher S. Karger
Language eng
Abstract Circulating monocytes and vascular endothelial cells (EC) interact in a complex and dynamic manner that varies between vascular beds. The objective of this study was twofold: to ascertain if monocytic cell adhesion to vascular endothelium differed between specific anatomic regions of the canine aorta, and to investigate the effect of known EC stimulators on monocytic cell adhesion to cells from these regions. Initial in vitro studies measuring adherence of U937 cells, a human monocytic cell line, to canine jugular vein and aortic EC monolayers revealed a dose-dependent increase in adhesion to EC stimulated with interleukin-1 (IL-1), lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA), or thrombin. While there was no regional difference in monocytic cell adherence to unstimulated LC in tissue culture, studies demonstrated greater monocytic cell adhesion to stimulated EC cultured from the distal versus proximal aorta. In organ culture, unstimulated adhesion of U937 cells or autologous monocytes was significantly greater to the distal aorta than the proximal aorta. Although monocytic cell adhesion to both the proximal and distal aorta increased with stimulation, the percentage increase in the proximal aorta, 1,086% with IL-1, 237% with PMA, 209% with LPS, and 174% with thrombin, was greater than in the distal aorta, demonstrating a significant functional difference in the endothelium from separate anatomic regions of a single vessel. This may have a direct relevance to the regional specificity of vascular disease.
Keyword Endothelial cell
Organ culture
Cytokine stimulation
Blood vessels
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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