Regional differences in platelet-derived growth factor production by the canine aorta

Madura, J.A., Kaufman, B.R., Margolin, D.A., Spencer, D.M., Fox, P.L. and Graham, L.M. (1996) Regional differences in platelet-derived growth factor production by the canine aorta. Journal of Vascular Research, 33 1: 53-61.

Author Madura, J.A.
Kaufman, B.R.
Margolin, D.A.
Spencer, D.M.
Fox, P.L.
Graham, L.M.
Title Regional differences in platelet-derived growth factor production by the canine aorta
Journal name Journal of Vascular Research   Check publisher's open access policy
ISSN 1018-1172
Publication date 1996-01
Sub-type Article (original research)
Volume 33
Issue 1
Start page 53
End page 61
Total pages 9
Place of publication Basel, Switzerland
Publisher S. Karger
Language eng
Formatted abstract
Platelet-derived growth factor (PDGF) is a potent mitogen and chemotactic agent which may be involved in the formation of proliferative lesions of the arterial system, such as intimal hyperplasia and atherosclerosis. To examine the regional variation in vessel wall production of this mitogen, PDGF production and PDGF A chain mRNA expression by normal arterial wall was studied as a function of vessel location. PDGF production by canine aortic segments was measured after 72 h in organ culture, revealing significantly more PDGF produced by the distal compared to proximal aorta at 77 ± 10 versus 14 ± 6 pg/cm 2/72 h (p < 0.05). Endothelial cells (EC) and smooth muscle cells (SMC), isolated from analogous aortic sites, were grown in tissue culture and the conditioned medium was assayed for PDGF. EC in vitro demonstrated a similar geographic trend in PDGF production (distal = 1,501 ± 389 pg/μg DNA/72 h, proximal = 759 ± 230 pg/μg DNA/72 h; p = 0.17). PDGF production by SMC in cell culture had a similar pattern with cells from the distal aorta producing 58 ± 28 pg PDGF/μg DNA/72 h, compared to cells from the proximal aorta producing 37 ± 15 pg PDGF/μg DNA/72 h (p = 0.13). Freshly harvested EC and SMC, isolated from the same aortic sites, were subjected to quantitation of PDGF mRNA levels using a coupled reverse transcriptase and polymerase chain reaction amplification method, with glyceraldehyde-phosphate dehydrogenase (GAPDH) as a control. The ratio of PDGF A chain:GAPDH mRNA was significantly greater in distal aortic SMC, 2.30 ± 0.99, compared to proximal aortic SMC, 1.27 ± 0.46 (p = 0.05), but was not significantly different between proximal and distal aortic EC (p = 0.86). These findings demonstrate significant regional differences in PDGF production in the normal canine aorta. Additionally, SMC are implicated as a significant contributor to the regional variation in PDGF production.
Keyword Platelet-derived growth factor
Endothelial cell
Smooth muscle cell
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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