Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux

Petty, Nicola K., Feltwell, Theresa, Pickard, Derek, Clare, Simon, Toribio, Ana L., Fookes, Maria, Roberts, Kevin, Monson, Rita, Nair, Satheesh, Kingsley, Robert A., Bulgin, Richard, Wiles, Siouxsie, Goulding, David, Keane, Thomas, Corton, Craig, Lennard, Nicola, Harris, David, Willey, David, Rance, Richard, Yu, Lu, Choudhary, Jyoti S., Churcher, Carol, Quail, Michael A., Parkhill, Julian, Frankel, Gad, Dougan, Gordon, Salmond, George P. C. and Thomson, Nicholas R. (2011) Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux. PLoS Pathogens, 7 4: e1002018.1-e1002018.15. doi:10.1371/journal.ppat.1002018

Author Petty, Nicola K.
Feltwell, Theresa
Pickard, Derek
Clare, Simon
Toribio, Ana L.
Fookes, Maria
Roberts, Kevin
Monson, Rita
Nair, Satheesh
Kingsley, Robert A.
Bulgin, Richard
Wiles, Siouxsie
Goulding, David
Keane, Thomas
Corton, Craig
Lennard, Nicola
Harris, David
Willey, David
Rance, Richard
Yu, Lu
Choudhary, Jyoti S.
Churcher, Carol
Quail, Michael A.
Parkhill, Julian
Frankel, Gad
Dougan, Gordon
Salmond, George P. C.
Thomson, Nicholas R.
Title Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux
Formatted title
Citrobacter rodentium is an Unstable Pathogen Showing Evidence of Significant Genomic Flux
Journal name PLoS Pathogens   Check publisher's open access policy
ISSN 1553-7366
Publication date 2011-04
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1002018
Open Access Status DOI
Volume 7
Issue 4
Start page e1002018.1
End page e1002018.15
Total pages 15
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2012
Language eng
Formatted abstract
Citrobacter rodentium is a natural mouse pathogen that causes attaching and effacing (A/E) lesions. It shares a common virulence strategy with the clinically significant human A/E pathogens enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) and is widely used to model this route of pathogenesis. We previously reported the complete genome sequence of C. rodentium ICC168, where we found that the genome displayed many characteristics of a newly evolved pathogen. In this study, through PFGE, sequencing of isolates showing variation, whole genome transcriptome analysis and examination of the mobile genetic elements, we found that, consistent with our previous hypothesis, the genome of C. rodentium is unstable as a result of repeat-mediated, large-scale genome recombination and because of active transposition of mobile genetic elements such as the prophages. We sequenced an additional C. rodentium strain, EX-33, to reveal that the reference strain ICC168 is representative of the species and that most of the inactivating mutations were common to both isolates and likely to have occurred early on in the evolution of this pathogen. We draw parallels with the evolution of other bacterial pathogens and conclude that C. rodentium is a recently evolved pathogen that may have emerged alongside the development of inbred mice as a model for human disease. © 2011 Petty et al.
Keyword Murine Colonic Hyperplasia
Enterohemorrhagic Escherichia-coli
Generalized Transducing Phage
Causative Agent
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Received: October 11, 2010; Accepted: February 18, 2011; Published: April 7, 2011 Article # e1002018

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 10 Jun 2011, 16:53:35 EST by Dr Nicola Petty on behalf of School of Chemistry & Molecular Biosciences