Functional genomic analysis of potato tuber life-cycle

Bachem, C., Van Der Hoeven, R., Lücker, J., Oomen, R., Casarini, E., Jacobsen, E. and Visser, R. (2000) Functional genomic analysis of potato tuber life-cycle. Potato Research, 43 4: 297-312. doi:10.1007/BF02360536


Author Bachem, C.
Van Der Hoeven, R.
Lücker, J.
Oomen, R.
Casarini, E.
Jacobsen, E.
Visser, R.
Title Functional genomic analysis of potato tuber life-cycle
Journal name Potato Research   Check publisher's open access policy
ISSN 0014-3065
1871-4528
Publication date 2000
Sub-type Article (original research)
DOI 10.1007/BF02360536
Volume 43
Issue 4
Start page 297
End page 312
Total pages 16
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Abstract Potato tuber life-cycle is composed of many individual developmental stages including tuber formation, tuber development, dormancy and sprouting. We have used cDNA-AFLP fingerprinting to analyse gene expression in 24 individual stages of development, over the period from stolon formation through sprouting. In addition to these developmental stages, different tissues were analysed to assess tissue specificity and various controls were incorporated to determine process specificity. In total around 18000 transcript derived cDNA fragments (TDFs) were visualised from which circa 2600 were included in a statistical analysis allowing general conclusions about gene expression during development. More than 200 process specific TDFs were isolated and sequenced throughout the potato tuber life-cycle. The sequence similarities of these TDFs to known genes give an insight into the kinds of processes occurring during tuberisation, dormancy and sprouting.
Keyword Gene expression
cDNA-AFLP
RNA-fingerprinting
Organogenesis
Tuberisation
Dormancy
Sprouting
Cluster analysis
Metabolic pathways
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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