Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-beta-D-glucopyranoside

Lücker, Joost, Bouwmeester, Harro J., Schwab, Wilfried, Blaas, Jan, van der Plas, Linus H. W. and Verhoeven, Harrie A. (2001) Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-beta-D-glucopyranoside. Plant Journal, 27 4: 315-324. doi:10.1046/j.1365-313x.2001.01097.x


Author Lücker, Joost
Bouwmeester, Harro J.
Schwab, Wilfried
Blaas, Jan
van der Plas, Linus H. W.
Verhoeven, Harrie A.
Title Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-beta-D-glucopyranoside
Journal name Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 2001-08
Sub-type Article (original research)
DOI 10.1046/j.1365-313x.2001.01097.x
Volume 27
Issue 4
Start page 315
End page 324
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract Petunia hybrida W115 was transformed with a Clarkia breweri S-linalool synthase cDNA (lis). Lis was expressed in all tissues analysed, and linalool was detected in leaves, sepals, corolla, stem and ovary, but not in nectaries, roots, pollen and style. However, the S-linalool produced by the plant in the various tissues is not present as free linalool, but was efficiently converted to non-volatile S-linalyl-β-D-glucopyranoside by the action of endogenous glucosyltransferase. The results presented demonstrate that monoterpene production can be altered by genetic modification, and that the compounds produced can be converted by endogenous enzymatic activity.
Keyword Petunia hybrida
Pathway engineering
Monoterpenoids
Monoterpene-glycoside
Liquid chromatography-tandem MS
S-linalool synthase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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