Development of a method for the determination of pulsatile growth hormone secretion in mice

Steyn, F. J., Huang, L., Ngo, S. T., Leong, J. W., Tan, H. Y., Xie, T. Y., Parlow, A. F., Veldhuis, J. D., Waters, M. J. and Chen, C. (2011) Development of a method for the determination of pulsatile growth hormone secretion in mice. Endocrinology, 152 8: 3165-3171. doi:10.1210/en.2011-0253


Author Steyn, F. J.
Huang, L.
Ngo, S. T.
Leong, J. W.
Tan, H. Y.
Xie, T. Y.
Parlow, A. F.
Veldhuis, J. D.
Waters, M. J.
Chen, C.
Title Development of a method for the determination of pulsatile growth hormone secretion in mice
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
1945-7170
Publication date 2011-05-17
Sub-type Article (original research)
DOI 10.1210/en.2011-0253
Volume 152
Issue 8
Start page 3165
End page 3171
Total pages 7
Place of publication Chevy Chase, MD, United States
Publisher The Endocrine Society
Collection year 2012
Language eng
Formatted abstract
Measures of pulsatile GH secretion require frequent collection and analysis of blood samples
at regular intervals. Due to blood volume constraints, repeat measures of circulating levels of
GH in mice remain challenging. Consequently, few observations exist in which the pulsatile
pattern of GH secretion in mice have been characterized. To address this, we developed a
technique for the collection and analysis of circulating levels of GH at regular and frequent
intervals in freely moving mice. This was achieved through the development of a sensitive assay
for the detection of GH in small (2μ) quantities of whole blood. The specificity and accuracy
of this assay was validated following guidelines established for single-laboratory validation as
specified by the International Union of Pure and Applied Chemistry. We incorporated an
established method for tail-clip blood sample collection to determine circulating levels of GH
secretion in 36 whole blood samples collected consecutively over a period of 6 h. Resulting
measures were characterized by peak secretion periods and interpulse stable baseline secretion
periods. Periods characterized by elevated whole blood GH levels consisted of multicomponent
peaks. Deconvolution analysis of resulting measures confirmed key parameters associated with
pulsatileGHsecretion.Weshow a striking decrease in pulsatileGHsecretion in mice after 12–18
h of fasting. This model is necessary to characterize the pulsatile profile of GH secretion in mice
and will significantly contribute to current attempts to clarify mechanisms that contribute to the
regulation of GH secretion.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published ahead of print May 17, 2011

 
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Created: Wed, 01 Jun 2011, 16:40:26 EST by Mr Frederik Steyn on behalf of School of Biomedical Sciences