Analysis of androgenic steroids in environmental waters by large-volume injection liquid chromatography tandem mass spectrometry

Backe, Will J., Ort, Christoph, Brewer, Alex J. and Field, Jennifer A. (2011) Analysis of androgenic steroids in environmental waters by large-volume injection liquid chromatography tandem mass spectrometry. Analytical Chemistry, 83 7: 2622-2630. doi:10.1021/ac103013h


Author Backe, Will J.
Ort, Christoph
Brewer, Alex J.
Field, Jennifer A.
Title Analysis of androgenic steroids in environmental waters by large-volume injection liquid chromatography tandem mass spectrometry
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 0003-2700
1520-6882
Publication date 2011-04-01
Sub-type Article (original research)
DOI 10.1021/ac103013h
Volume 83
Issue 7
Start page 2622
End page 2630
Total pages 9
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2012
Language eng
Abstract A new method was developed for the analysis of natural and synthetic androgenic steroids and their selected metabolites in aquatic environmental matrixes using direct large-volume injection (LVI) high-performance liquid chromatography (HPLC) tandem mass spectrometry (MS/MS). Method accuracy ranged from 87.6 to 108% for analytes with well-matched internal standards. Precision, quantified by relative standard deviation (RSD), was less than 12%. Detection limits for the method ranged from 1.2 to 360 ng/L. The method was demonstrated on a series of 1 h composite wastewater influent samples collected over a day with the purpose of assessing temporal profiles of androgen loads in wastewater. Testosterone, androstenedione, boldenone, and nandrolone were detected in the sample series at concentrations up to 290 ng/L and loads up to 535 mg/h. Boldenone, a synthetic androgen, had a temporal profile that was strongly correlated to testosterone, a natural human androgen, suggesting its source may be endogenous. An analysis of the sample particulate fraction revealed detectable amounts of sorbed testosterone and androstenedione. Androstenedione sorbed to the particulate fraction accounted for an estimated 5 to 7% of the total androstenedione mass. © 2011 American Chemical Society.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Advanced Water Management Centre Publications
 
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Created: Wed, 01 Jun 2011, 09:27:46 EST by Hong Lee on behalf of Advanced Water Management Centre