The viscoelastic, hyperelastic and scale dependent behaviour of freshly excised individual skin layers

Crichton, Michael L., Donose, Bogdan C., Chen, Xianfeng, Raphael, Anthony P., Huang, Han and Kendal, Mark A.F. (2011) The viscoelastic, hyperelastic and scale dependent behaviour of freshly excised individual skin layers. Biomaterials, 32 20: 4670-4681. doi:10.1016/j.biomaterials.2011.03.012

Author Crichton, Michael L.
Donose, Bogdan C.
Chen, Xianfeng
Raphael, Anthony P.
Huang, Han
Kendal, Mark A.F.
Title The viscoelastic, hyperelastic and scale dependent behaviour of freshly excised individual skin layers
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2011-07
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2011.03.012
Volume 32
Issue 20
Start page 4670
End page 4681
Total pages 12
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2012
Language eng
Abstract Micro-devices using mechanical means to target skin for improved drug and vaccine delivery have great promise for improved clinical healthcare. Fully realizing this promise requires a greater understanding of key micro-biomechanical properties for each of the different skin layers – that are both the mechanical barriers and biological targets of these devices. Here, we performed atomic force microscopy indentation on a micro-nano scale to quantify separately, in fresh mouse skin, the viscous and elastic behaviour of the stratum corneum, viable epidermis and dermis. By accessing each layer directly, we examined the response to nanoindentation at sub-cellular and bulk-cellular scale. We found that the dermis showed greatest mechanical stiffness (elastic moduli of 7.33–13.48 MPa for 6.62 μm and 1.90 μm diameter spherical probes respectively). In comparison, the stratum corneum and viable epidermis were weaker at 0.75–1.62 MPa and 0.49–1.51 MPa respectively (again with the lower values resulting from indentations with the large probe 6.62 μm). The living cell layer of the epidermis (viable epidermis) showed greatest viscoelasticity – almost fully relaxing from shallow indentation – whilst the other layers reached a plateau after relaxing by around 40%. With small scale (sub-micron) AFM indentation, we directly determined the effects of different layer constituents – in particular, the dermis showed that some indents contacted collagen fibrils and others contacted ground substance/cellular areas. This work has far reaching implications for the design of micro-devices using mechanical means to deliver drugs or vaccines into the skin; providing key characterized mechanical property values for each constituent of the target delivery material.
Keyword Skin
Mechanical properties
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mechanical & Mining Engineering Publications
Official 2012 Collection
Advanced Water Management Centre Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 56 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 56 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 26 May 2011, 07:01:22 EST by Dr Bogdan Donose on behalf of Advanced Water Management Centre