Induction of calcitonin receptors by lα, 25- dihydroxyvitamin D3 in osteoclast-like multinucleated cells formed from mouse bone marrow cells

Takahashi, Naoyuki, Akatsu, Takuhiko, Sasaki, Takahisa, Nicholson, Geoff C., Moseley, Jane M., Martin, T. John and Suda, Tatsuo (1988) Induction of calcitonin receptors by lα, 25- dihydroxyvitamin D3 in osteoclast-like multinucleated cells formed from mouse bone marrow cells. Endocrinology, 123 3: 1504-1510.


Author Takahashi, Naoyuki
Akatsu, Takuhiko
Sasaki, Takahisa
Nicholson, Geoff C.
Moseley, Jane M.
Martin, T. John
Suda, Tatsuo
Title Induction of calcitonin receptors by lα, 25- dihydroxyvitamin D3 in osteoclast-like multinucleated cells formed from mouse bone marrow cells
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
1945-7170
Publication date 1988-09
Sub-type Article (original research)
Volume 123
Issue 3
Start page 1504
End page 1510
Total pages 7
Place of publication Chevy Chase, MD, United States
Publisher The Endocrine Society
Language eng
Formatted abstract
We have developed a mouse marrow culture system, in which multinucleated cells (MNCs) are formed within 6-8 days. These MNCs showed several characteristics of osteoclasts, including tartrate-resistant acid phosphatase (TRACP) and the ability to resorb calcified dentine. lα,25-Dihydroxyvitamin D3 [lα,25(OH)2D3] stimulated the formation of TRACPpositive MNCs, and salmon calcitonin (CT) inhibited it. In this study, we examined whether the TRACP-positive MNCs formed from mouse marrow cells possess CT receptors, another typical characteristic of osteoclasts. Mouse marrow cells cultured for 8 days with 10 nM lα,25(OH)2D3 and freshly isolated authentic mouse osteoclasts were incubated with [125I]-salmon CT in the presence or absence of excess amounts of unlabeled CT, stained for TRACP, and processed for autoradiography. The [125I]-CT exclusively bound to TRACP-positive mononuclear cells and MNCs formed in the lα,25(OH)2D3-treated cultures and also to isolated mouse osteoclasts. Both [125I]-CT binding and TRACP activity were induced simultaneously on mononuclear cells on day 3 in the cultures treated with lα,25(OH)2D3. CT markedly stimulated cAMP production in the lα,25(OH)2D3-treated cultures. The CT-dependent cAMP production increased in parallel with the increase in the number of TRACP-positive MNCs formed. Neither freshly isolated marrow cells nor cells cultured without lα,25(OH)2D3 showed CT-induced cAMP accumulation. Furthermore, CT induced cytoplasmic contraction of both marrow- derived MNCs and isolated osteoclasts. These results clearly indicate that lα,25(OH)2D3 induces TRACP activity and CT receptors almost simultaneously in mouse marrow cultures, and the MNCs formed in vitro respond to CT as authentic osteoclasts do.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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