Biphasic effect of calcitonin on tartrate-resistant acid phosphatase activity in isolated rat osteoclasts

Yumita, S., Nicholson, G.C., Rowe, D.J., Kent, G.N. and Martin, T.J. (1991) Biphasic effect of calcitonin on tartrate-resistant acid phosphatase activity in isolated rat osteoclasts. Journal of Bone and Mineral Research, 6 6: 591-597.


Author Yumita, S.
Nicholson, G.C.
Rowe, D.J.
Kent, G.N.
Martin, T.J.
Title Biphasic effect of calcitonin on tartrate-resistant acid phosphatase activity in isolated rat osteoclasts
Journal name Journal of Bone and Mineral Research   Check publisher's open access policy
ISSN 0884-0431
1523-4681
Publication date 1991-06
Sub-type Article (original research)
Volume 6
Issue 6
Start page 591
End page 597
Total pages 7
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Tartrate-resistant acid phosphatase (TRAP) has been implicated as being involved in osteoclastic bone resorption, and calcitonin (CT) is known to inhibit the resorptive process. This study investigates the kinetics of CT action on TRAP activity in isolated rat osteoclasts using both biochemical and quantitative cytochemical methods. The latter technique has been developed to detect very small changes in intracellular TRAP activity at the single-cell level. The biochemical study showed that 10−9 M salmon CT (sCT) decreased TRAP activity in medium throughout the experimental period; TRAP activity in the cells was increased during the first 2 h but subsequently declined and was decreased to a significant level at 6 h. TRAP activity in sCT-treated osteoclasts measured by the cytochemical method showed significant increases within the first hour. This response was dose dependent between 1016 and 10−11 M sCT with EC50 at 8 × 10−14 M. After 1 h, the initial increase in intracellular TRAP activity in CT-treated osteoclasts was followed by a decline to below control levels, reaching statistical significance at 9 h. Treatment with forskolin (10−5 M) showed a similar trend, suggesting that this response is mediated by cyclic AMP-regulated phosphorylation events. From these results, we conclude that CT has two actions on TRAP in isolated rat osteoclasts: the first to inhibit its release, the second to inhibit its synthesis and/or increase its degradation.
Keyword Parathyroid hormone
Bone resorption
Cytochemical bioassay
Lysosomal enzymes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 3 December 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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