Evidence that protein kinase-A, calcium-calmodulin kinase and cytoskeletal proteins are involved in osteoclast retraction induced by calcitonin

Zheng, M.H., Wood, D.J., Papadimitriou, J.M. and Nicholson, G.C. (1992) Evidence that protein kinase-A, calcium-calmodulin kinase and cytoskeletal proteins are involved in osteoclast retraction induced by calcitonin. Experimental and Molecular Pathology, 57 2: 105-115. doi:10.1016/0014-4800(92)90002-S


Author Zheng, M.H.
Wood, D.J.
Papadimitriou, J.M.
Nicholson, G.C.
Title Evidence that protein kinase-A, calcium-calmodulin kinase and cytoskeletal proteins are involved in osteoclast retraction induced by calcitonin
Journal name Experimental and Molecular Pathology   Check publisher's open access policy
ISSN 0014-4800
1096-0945
Publication date 1992-10
Sub-type Article (original research)
DOI 10.1016/0014-4800(92)90002-S
Volume 57
Issue 2
Start page 105
End page 115
Total pages 11
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Abstract Calcitonin is a direct inhibitor of osteoclastic activity. Osteoclast retraction is readily induced by calcitonin and it is possible that calcitonin-induced inhibition of bone resorption is in part due to this effect. However, little is known of the mechanisms of this action. In these studies, we have investigated the intracellular signalling pathway of calcitonin-induced osteoclast retraction using cultures of freshly isolated rat osteoclasts. The spread area occupied by single Giemsa-stained rat osteoclasts was measured in vitro by a computer imaging analysis system and used as a quantitative parameter for calculating the degree of osteoclast retraction in response to various agents. Our results show that cAMP may be an important second messenger in the reaction of osteoclasts to calcitonin. Moreover, both protein kinase-A and calcium/calmodulin-dependent protein kinase are involved in the osteoclast retraction induced by this hormone, while cytoskeletal proteins are required for the process to occur.
Keyword Parathyroid hormone
Rat osteoclasts
Bone resorption
Invitro
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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