Abundant calcitonin receptors in isolated rat osteoclasts. Biochemical and autoradiographic characterization

Nicholson, G.C., Moseley, J.M., Sexton, P.M., Mendelsohn, F.A.O. and Martin, T.J. (1986) Abundant calcitonin receptors in isolated rat osteoclasts. Biochemical and autoradiographic characterization. Journal of Clinical Investigation, 78 2: 355-360.


Author Nicholson, G.C.
Moseley, J.M.
Sexton, P.M.
Mendelsohn, F.A.O.
Martin, T.J.
Title Abundant calcitonin receptors in isolated rat osteoclasts. Biochemical and autoradiographic characterization
Journal name Journal of Clinical Investigation
Publication date 1986-08
Sub-type Article
DOI 10.1172/JCI112584
Volume number 78
Issue number 2
ISSN 0021-9738;1558-8238
Start page 355
End page 360
Total pages 6
Place of publication Ann Arbor, MI, United States
Publisher American Society for Clinical Investigation
Language eng
Formatted abstract Calcitonin receptors have been characterized for the first time in isolated osteoclasts. These receptors have been demonstrated by autoradiographic and biochemical methods, and the cells have also been shown to respond to calcitonin with a dose-dependent increase in cyclic AMP. The receptors in rat osteoclasts are specific and of high affinity (dissociation constant, K(d), 1 to 6 x 10-10 M), and are present in greater numbers than in any cell previously studied (>106 per cell). Chemical cross-linking of 125I-labeled salmon calcitonin to osteoclasts using disuccinimidyl suberate resulted in identification of a receptor component with a relative molecular weight of 80,000-90,000. By counting grains in autoradiographic experiments, we found that >80% of specifically bound radioactivity was associated with multinucleate osteoclasts and the remainder was associated with mononuclear cells that are not osteoblasts, but that may be osteoclast precursors.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article
Collection: School of Medicine Publications
 
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