Human placental calcitonin receptors

Nicholson, G.C., Dsantos, C.S., Evans, T., Moseley, J.M., Kemp, B.E., Michelangeli, V.P. and Martin, T.J. (1988) Human placental calcitonin receptors. Biochemical Journal, 250 3: 877-882.

Author Nicholson, G.C.
Dsantos, C.S.
Evans, T.
Moseley, J.M.
Kemp, B.E.
Michelangeli, V.P.
Martin, T.J.
Title Human placental calcitonin receptors
Journal name Biochemical Journal   Check publisher's open access policy
ISSN 0264-6021
1470-8728
Publication date 1988-03
Sub-type Article (original research)
Volume 250
Issue 3
Start page 877
End page 882
Total pages 6
Place of publication London, United Kingdom
Publisher Portland Press
Language eng
Formatted abstract
Receptors for the hypocalcaemic hormone, calcitonin (CT), have been identified in a membrane fraction prepared from term human placentae. Binding of 125I-labelled salmon CT ( 125I-sCT) to the membranes was time- and temperature-dependent, saturable (B(max) 58 ± 11 fmol/mg of protein), of high affinity (K(d) 80 ± 21 pM) and poorly reversible. Species-specific CTs and CT analogues competed for 125I-sCT binding with potencies proportional to their known biological potencies. Various unrelated peptide hormones did not compete, indicating that receptor binding was specific for CT. Photoaffinity labelling using a derivatized biologically active sCT analogue, [Arg 11,18,3-nitrophenylazide-Lys 14]sCT, identified a receptor component of M(r) ~ 85000, comparable with findings in osteoclasts and other target cells. The presence of CT receptors in the human placenta supports other evidence that CT may have a role in the regulation of placental function.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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