Adenoviral down-regulation of osteopontin inhibits human osteoclast differentiation in vitro

Aitken, Cathy J., Hodge, Jason M. and Nicholson, Geoffrey C. (2004) Adenoviral down-regulation of osteopontin inhibits human osteoclast differentiation in vitro. Journal of Cellular Biochemistry, 93 5: 896-903. doi:10.1002/jcb.20247

Author Aitken, Cathy J.
Hodge, Jason M.
Nicholson, Geoffrey C.
Title Adenoviral down-regulation of osteopontin inhibits human osteoclast differentiation in vitro
Journal name Journal of Cellular Biochemistry   Check publisher's open access policy
ISSN 0730-2312
Publication date 2004-11
Sub-type Article (original research)
DOI 10.1002/jcb.20247
Volume 93
Issue 5
Start page 896
End page 903
Total pages 8
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract Although osteopontin (OPN) is highly expressed in osteoclasts, OPN-deficient mice have a near-normal bone phenotype and its role in osteoclast differentiation and function remains uncertain. We used an adenoviral OPN-antisense vector (AdOPN-AS) to down-regulate OPN expression in a human in vitro osteoclastogenesis model employing CFU-GM precursors treated with RANKL and M-CSF. Cultures infected with AdOPN-AS showed reduced secretion of OPN compared to cultures infected with a control adenoviral vector expressing β-galactosidase. Infection with AdOPN-AS co-incident with exposure to RANKL was associated with substantial (approximately 50%) inhibition of osteoclast formation with a concomitant reduction in dentine resorption. There was also a small reduction in the size of generated osteoclasts but no significant effect on the size of resorption pits/tracks nor on the amount of resorption per osteoclast. When the cultures were infected with AdOPN-AS after 4 days exposure to RANKL only minor effects on osteoclastogenesis were seen. Our data demonstrate that early down-regulation of OPN in vitro inhibits human osteoclastogenesis. Since mice totally lacking OPN do not have reduced osteoclast numbers our results imply the existence in vivo of an alternative molecular pathway(s).
Keyword Osteopontin
Osteoclast differentiation
Adenoviral gene transfer
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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