A pH-responsive interface derived from resilin-mimetic protein Rec1-resilin

Truong, MY, Dutta, NK, Choudhury, NR, Kim, M, Elvin, CM, Hill, AJ, Thierry, B and Vasilev, K (2010) A pH-responsive interface derived from resilin-mimetic protein Rec1-resilin. Biomaterials, 31 15: 4434-4446. doi:10.1016/j.biomaterials.2010.02.019

Author Truong, MY
Dutta, NK
Choudhury, NR
Kim, M
Elvin, CM
Hill, AJ
Thierry, B
Vasilev, K
Title A pH-responsive interface derived from resilin-mimetic protein Rec1-resilin
Formatted title
A pH-responsive interface derived from resilin-mimetic protein Rec1-resilin
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2010-05
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2010.02.019
Volume 31
Issue 15
Start page 4434
End page 4446
Total pages 13
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
In this investigation, for the first time we report the effects of pH on the molecular orientation, packing density, structural properties, adsorption characteristics and viscoelastic behaviour of resilin-mimetic protein rec1-resilin at the solid-liquid interface using quartz crystal microbalance with dissipation monitoring (QCM-D) and surface plasmon resonance (SPR) spectroscopy. QCM-D and SPR data confirm that the binding ability of rec1-resilin on a substrate is strongly pH-dependent the protein packing density on a gold surface is calculated to be 4.45 × 1013 per cm2 at the isoelectric point (IEP ∼ 4.9), 8.79 × 1011 per cm2 at pH 2 and 9.90 × 1011 per cm2 at pH 12, respectively. Our findings based on the thickness, dissipation and viscoelasticity of the rec1-resilin adlayer also indicate that it is adsorbed onto the gold substrate with different orientation depending on pH, such as back-on adsorption at acidic pH of 2, compact end-on bilayer adsorption at the IEP and side-on at high alkaline pH of 12. When rec1-resilin is 'pinned' to the substrate at IEP and subsequently exposed to an electrolyte solution adjusted to different pH, it switches from a compact globular conformation of the bio-macromolecule at the IEP to a coil conformation at pH between IEP to IED (IED = pKa value of tyrosine amino acid residue) and an extended coil conformation at pH > IED. This transformation from globule to coil to extended coil conformation is kinetically fast, robust and completely reversible. Such responsive surfaces created using 'smart' biomimetic rec1-resilin have the potential to find applications in many areas including biotechnology, medicine, sensors, controlled drug delivery systems and engineering.
© 2010 Elsevier Ltd.
Keyword Biomimetic material
Protein adsorption
Quartz crystal microbalance
Recombinant protein
Responsive interface
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
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Created: Mon, 11 Apr 2011, 10:44:10 EST by Ms Misook Kim on behalf of School of Chemistry & Molecular Biosciences