A highly elastic tissue sealant based on photopolymerised gelatin

Elvin, CM, Vuocolo, T, Brownlee, AG, Sando, L, Huson, MG, Liyou, NE, Stockwell, PR, Lyons, RE, Kim, M, Edwards, GA, Johnson, G, McFarland, GA, Ramshaw, JAM and Werkmeister, JA (2010) A highly elastic tissue sealant based on photopolymerised gelatin. Biomaterials, 31 32: 8323-8331. doi:10.1016/j.biomaterials.2010.07.032

Author Elvin, CM
Vuocolo, T
Brownlee, AG
Sando, L
Huson, MG
Liyou, NE
Stockwell, PR
Lyons, RE
Kim, M
Edwards, GA
Johnson, G
McFarland, GA
Ramshaw, JAM
Werkmeister, JA
Title A highly elastic tissue sealant based on photopolymerised gelatin
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2010.07.032
Volume 31
Issue 32
Start page 8323
End page 8331
Total pages 9
Place of publication Amsterdam, The Netherlands
Publisher Elsevier B V
Collection year 2011
Language eng
Formatted abstract
Gelatin is widely used as a medical biomaterial because it is readily available, cheap, biodegradable and demonstrates favourable biocompatibility. Many applications require stabilisation of the biomaterial by chemical crosslinking, and this often involves derivatisation of the protein or treatment with cytotoxic crosslinking agents. We have previously shown that a facile photochemical method, using blue light, a ruthenium catalyst and a persulphate oxidant, produces covalent di-tyrosine crosslinks in resilin and fibrinogen to form stable hydrogel biomaterials. Here we show that various gelatins can also be rapidly crosslinked to form highly elastic (extension to break >650%) and adhesive (stress at break >100. kPa) biomaterials. Although the method does not require derivatisation of the protein, we show that when the phenolic (tyrosine-like) content of gelatin is increased, the crosslinked material becomes resistant to swelling, yet retains considerable elasticity and high adhesive strength. The reagents are not cytotoxic at the concentration used in the photopolymerisation reaction. When tested in vivo in sheep lung, the photopolymerised gelatin effectively sealed a wound in lung tissue from blood and air leakage, was not cytotoxic and did not produce an inflammatory response. The elastic properties, thermal stability, speed of curing and high tissue adhesive strength of this photopolymerised gelatin, offer considerable improvement over current surgical tissue sealants.
© 2010 Published by Elsevier Ltd
Keyword Gelatin
Tissue sealant
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
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Created: Mon, 11 Apr 2011, 10:39:15 EST by Ms Misook Kim on behalf of School of Chemistry & Molecular Biosciences