Molecular cloning of a novel fimbrin-like cDNA from Arabidopsis thaliana

McCurdy, David W. and Kim, Misook (1998) Molecular cloning of a novel fimbrin-like cDNA from Arabidopsis thaliana. Plant Molecular Biology, 36 1: 23-31. doi:10.1023/A:1005884112192


Author McCurdy, David W.
Kim, Misook
Title Molecular cloning of a novel fimbrin-like cDNA from Arabidopsis thaliana
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
1573-5028
Publication date 1998-03-04
Sub-type Article (original research)
DOI 10.1023/A:1005884112192
Volume 36
Issue 1
Start page 23
End page 31
Total pages 9
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Formatted abstract
Fimbrin is a 68-70 kDa actin-bundling protein in animal cells and lower eukaryotes that participates in diverse morphogenetic processes by cross-linking actin filaments into bundles. Here we report the cloning by degenerate polymerase chain reaction (PCR) of ATF1M1, a 2.3 kb cDNA from Arabidopsis thaliana that codes for a novel 76 kDa fimbrin-like polypeptide (AtFim1). The predicted sequence of AtFim1 shares ca. 40% identify as a putative actin-binding domain in fimbrins and in a larger family of actin cross-linking proteins. Preceding the tandem repeats at the amino terminus of AtFim1 is a single-EF-hand-like domain with moderate homology to calmodulin-like calcium-binding proteins. AtFim1 differs from non-plant fimbrins, however, in that it contains an extended carboxy-terminal tail of ca. 65 amino acids. ATF1M1 is encoded by a single gene, although sequencing of two partial fimbrin-like expressed sequence tag (EST) clones indicates that Arabidopsis contains at least two fimbrin-like proteins. Northern blot analysis and reverse-transcription PCR (RT-PCR) demonstrated that ATF1M1 is expressed in all major organs examined (roots, leaves, stems, flowers and siliques). This is the first report of the cloning of a full length plant gene that encodes a putative actin filament-binding protein.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Mon, 11 Apr 2011, 10:19:30 EST by Ms Misook Kim on behalf of School of Chemistry & Molecular Biosciences