Regulation of monocyte subset systemic levels by distinct chemokine receptors controls post-ischaemic neovascularization

Cochain, Clément, Rodero, Mathieu P., Vilar, José, Récalde, Alice, Richart, Adèle L., Loinard, Céline, Zouggari, Yasmine, Guérin, Coralie, Duriez, Micheline, Combadière, Behazine, Poupel, Lucie, Lévy, Bernard I., Mallat, Ziad, Combadière, Christophe and Silvestre, Jean-Sébastien (2010) Regulation of monocyte subset systemic levels by distinct chemokine receptors controls post-ischaemic neovascularization. Cardiovascular Research, 88 1: 186-195. doi:10.1093/cvr/cvq153


Author Cochain, Clément
Rodero, Mathieu P.
Vilar, José
Récalde, Alice
Richart, Adèle L.
Loinard, Céline
Zouggari, Yasmine
Guérin, Coralie
Duriez, Micheline
Combadière, Behazine
Poupel, Lucie
Lévy, Bernard I.
Mallat, Ziad
Combadière, Christophe
Silvestre, Jean-Sébastien
Title Regulation of monocyte subset systemic levels by distinct chemokine receptors controls post-ischaemic neovascularization
Journal name Cardiovascular Research   Check publisher's open access policy
ISSN 0008-6363
1755-3245
Publication date 2010-10-01
Sub-type Article (original research)
DOI 10.1093/cvr/cvq153
Volume 88
Issue 1
Start page 186
End page 195
Total pages 10
Editor Hans Michael Piper
David Garcia-Dorado
Place of publication Oxford, U.K
Publisher Oxford University Press
Collection year 2011
Language eng
Formatted abstract
Aims Monocyte systemic levels are known to be a major determinant of ischaemic tissue revascularization, but the mechanisms mediating mobilization of different monocyte subsets—Ly6Chi and Ly6Clo—to the blood and their respective role in post-ischaemic neovascularization are not clearly understood. Here, we hypothesized that distinct chemokine/chemokine receptor pathways, namely CCL2/CCR2, CX3CL1/CX3CR1, and CCL5/CCR5, differentially control monocyte subset systemic levels, and might thus impact post-ischaemic vessel growth.

Methods and results In a model of murine hindlimb ischaemia, both Ly6Chi and Ly6Clo monocyte circulating levels were increased after femoral artery ligation. CCL2/CCR2 activation enhanced blood Ly6Chi and Ly6Clo monocyte counts, although the opposite effect was seen in mice with CCL2 or CCR2 deficiency. CX3CL1/CX3CR1 strongly impacted Ly6Clo monocyte levels, whereas CCL5/CCR5 had no role. Only CCL2/CCR2 signalling influenced neovascularization, which was increased in mice overexpressing CCL2, whereas it markedly decreased in CCL2−/− mice. Moreover, adoptive transfer of Ly6Chi—but not Ly6Clo—monocytes enhanced vessel growth and blood flow recovery.

Conclusion Altogether, our data demonstrate that regulation of proangiogenic Ly6Chi monocytes systemic levels by CCL2/CCR2 controls post-ischaemic vessel growth, whereas Ly6Clo monocytes have no major role in this setting.
Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2010.
Keyword Ischaemia
Angiogenesis
Arteriogenesis
Inflammation
Monocyte subset
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Non HERDC
 
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Created: Fri, 08 Apr 2011, 15:43:45 EST by Dr Mathieu Rodero on behalf of !NON-HERDC