Sodium-dependent uptake of inorganic phosphate by the intracellular malaria parasite

Saliba, Kevin J., Martin, Rowena E., Broer, Angelika, Henry, Roselani I., McCarthy, C. Siobhan, Downie, Megan J., Allen, Richard J. W., Mullin, Kylie A., McFadden, Geoffrey I., Broer, Stefan and Kirk, Kiaran (2006) Sodium-dependent uptake of inorganic phosphate by the intracellular malaria parasite. Nature, 443 7111: 582-585. doi:10.1038/nature05149


Author Saliba, Kevin J.
Martin, Rowena E.
Broer, Angelika
Henry, Roselani I.
McCarthy, C. Siobhan
Downie, Megan J.
Allen, Richard J. W.
Mullin, Kylie A.
McFadden, Geoffrey I.
Broer, Stefan
Kirk, Kiaran
Title Sodium-dependent uptake of inorganic phosphate by the intracellular malaria parasite
Journal name Nature   Check publisher's open access policy
ISSN 0028-0836
1476-4687
Publication date 2006-10-05
Sub-type Article (original research)
DOI 10.1038/nature05149
Volume 443
Issue 7111
Start page 582
End page 585
Total pages 4
Place of publication London, U.K.
Publisher Nature Publishing Group
Language eng
Formatted abstract
As the malaria parasite, Plasmodium falciparum, grows within its host erythrocyte it induces an increase in the permeability of the erythrocyte membrane to a range of low-molecular-mass solutes, including Na+ and K+ (ref. 1). This results in a progressive increase in the concentration of Na+ in the erythrocyte cytosol. The parasite cytosol has a relatively low Na+ concentration and there is therefore a large inward Na+ gradient across the parasite plasma membrane. Here we show that the parasite exploits the Na+ electrochemical gradient to energize the uptake of inorganic phosphate (Pi), an essential nutrient. Pi was taken up into the intracellular parasite by a Na+-dependent transporter, with a stoichiometry of 2Na +:1Pi and with an apparent preference for the monovalent over the divalent form of Pi. A Pi transporter (PfPiT) belonging to the PiT family was cloned from the parasite and localized to the parasite surface. Expression of PfPiT in Xenopus oocytes resulted in Na +-dependent Pi uptake with characteristics similar to those observed for Pi uptake in the parasite. This study provides new insight into the significance of the malaria-parasite-induced alteration of the ionic composition of its host cell. © 2006 Nature Publishing Group.
Keyword Xenopus-laevis oocytes
Plasmodium-falciparum
Membrane-transport
Acid
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Published under Letters

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Thu, 07 Apr 2011, 14:31:39 EST by Susan Allen on behalf of Institute for Molecular Bioscience