Acid extrusion from the intraerythrocytic malaria parasite is not via a Na+/H+ exchanger

Spillman, Natalie J., Allen, Richard J. W. and Kirk, Kiaran (2008) Acid extrusion from the intraerythrocytic malaria parasite is not via a Na+/H+ exchanger. Molecular and Biochemical Parasitology, 162 1: 96-99.


Author Spillman, Natalie J.
Allen, Richard J. W.
Kirk, Kiaran
Title Acid extrusion from the intraerythrocytic malaria parasite is not via a Na+/H+ exchanger
Formatted title Acid extrusion from the intraerythrocytic malaria parasite is not via a Na+/H+ exchanger
Journal name Molecular and Biochemical Parasitology   Check publisher's open access policy
ISSN 0166-6851
1872-9428
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1016/j.molbiopara.2008.07.001
Volume 162
Issue 1
Start page 96
End page 99
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract The intraerythrocytic malaria parasite, Plasmodium falciparum maintains an intracellular pH (pHi) of around 7.3. If subjected to an experimentally imposed acidification the parasite extrudes H+, thereby undergoing a pHi recovery. In a recent study, Bennett et al. [Bennett TN, Patel J, Ferdig MT, Roepe PD. P. falciparum Na+/H+ exchanger activity and quinine resistance. Mol Biochem Parasitol 2007;153:48-58] used the H+ ionophore nigericin, in conjunction with an acidic medium, to acidify the parasite cytosol, and then used bovine serum albumin (BSA) to scavenge the nigericin from the parasite membrane. The ensuing Na+-dependent pHi recovery, seen following an increase in the extracellular pH, was attributed to a plasma membrane Na+/H+ exchanger. This is at odds with previous reports that the primary H+ extrusion mechanism in the parasite is a plasma membrane V-type H+-ATPase. Here we present evidence that the Na+-dependent efflux of H+ from parasites acidified using nigericin/BSA is attributable to Na+/H+ exchange via residual nigericin remaining in the parasite plasma membrane, rather than to endogenous transporter activity.
© 2008 Elsevier B.V. All rights reserved.
Keyword Malaria
Plasmodium falciparum
Quinine
Drug resistance
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published under Short Communications

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Thu, 07 Apr 2011, 14:18:55 EST by Susan Allen on behalf of Institute for Molecular Bioscience