Na+-dependent acid efflux from P. falciparum: PfNHE or residual nigericin

Spillman, Natalie J., Allen, Richard J. W. and Kirk, Kiaran (2009) Na+-dependent acid efflux from P. falciparum: PfNHE or residual nigericin. Molecular and Biochemical Parasitology, 166 1: 3-3. doi:10.1016/j.molbiopara.2009.02.013


Author Spillman, Natalie J.
Allen, Richard J. W.
Kirk, Kiaran
Title Na+-dependent acid efflux from P. falciparum: PfNHE or residual nigericin
Journal name Molecular and Biochemical Parasitology   Check publisher's open access policy
ISSN 0166-6851
1872-9428
Publication date 2009-07
Sub-type Letter to editor, brief commentary or brief communication
DOI 10.1016/j.molbiopara.2009.02.013
Volume 166
Issue 1
Start page 3
End page 3
Total pages 1
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Whilst recognizing that there were methodological differences between the two studies we would point out that: (i) In both the original study by Roepe and coworkers, and our own, parasites recovered from an ammonium-prepulse-induced acidification using a Na+-independent mechanism, attributable to a plasma membrane V-type H+-ATPase. The Na+-dependent recovery, which we have attributed to residual nigericin, was in both studies seen only in parasites that had been acidified using nigericin-BSA. (ii) In a very recent study using methodology similar to that used by Roepe and colleagues (single cell microscopy, with perfusion) Nkrumah et al. found that in parasites expressing differing levels of PfNHE, quinine sensitivity varied without there being any significant variation in pHcyt and, furthermore, that for parasites acidified using nigericin-BSA the rate of recovery of pHcyt varied widely from experiment to experiment. As noted by these authors, the latter observation is consistent with there having been “differing residual amounts of nigericin in the parasite plasma membrane”.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Letter to editor, brief commentary or brief communication
Collection: Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 07 Apr 2011, 14:15:15 EST by Susan Allen on behalf of Institute for Molecular Bioscience