Plasmodium falciparum culture: The benefits of shaking

Allen, Richard J. W. and Kirk, Kiaran (2010) Plasmodium falciparum culture: The benefits of shaking. Molecular and Biochemical Parasitology, 169 1: 63-65. doi:10.1016/j.molbiopara.2009.09.005

Author Allen, Richard J. W.
Kirk, Kiaran
Title Plasmodium falciparum culture: The benefits of shaking
Formatted title
Plasmodium falciparum culture: The benefits of shaking
Journal name Molecular and Biochemical Parasitology   Check publisher's open access policy
ISSN 0166-6851
Publication date 2010-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.molbiopara.2009.09.005
Volume 169
Issue 1
Start page 63
End page 65
Total pages 3
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Abstract Despite evidence that the suspension of malaria cultures leads to improved parasite growth, the practice of culturing the parasite under static conditions remains widespread. Here, extending previous work, we have quantified the favourable effects of continuous agitation on three indices of culture growth: (i) parasite yield, (ii) culture synchrony after a synchronisation procedure, and (iii) the prevalence of multiple infections. In addition, we show that under continuous suspension, the time taken for genetically altered parasites to re-populate cultures post-transfection is dramatically reduced.© 2009 Elsevier B.V. All rights reserved.
Keyword Culture
Transfected parasites
Multiply infected erythrocytes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Published under Short technical reports. Available online 17 September 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
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Created: Thu, 07 Apr 2011, 14:10:03 EST by Susan Allen on behalf of Institute for Molecular Bioscience