Seminal plasma proteins do not consistently improve fertility after cervical insemination of ewes with non-sorted or sex-sorted frozen-thawed ram spermatozoa

Leahy, T., Evans, G., Maxwell, W. M. C. and Marti, J. I. (2010) Seminal plasma proteins do not consistently improve fertility after cervical insemination of ewes with non-sorted or sex-sorted frozen-thawed ram spermatozoa. Reproduction Fertility and Development, 22 4: 606-612. doi:10.1071/RD09207

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Author Leahy, T.
Evans, G.
Maxwell, W. M. C.
Marti, J. I.
Title Seminal plasma proteins do not consistently improve fertility after cervical insemination of ewes with non-sorted or sex-sorted frozen-thawed ram spermatozoa
Journal name Reproduction Fertility and Development   Check publisher's open access policy
ISSN 1031-3613
1448-5990
Publication date 2010
Sub-type Article (original research)
DOI 10.1071/RD09207
Volume 22
Issue 4
Start page 606
End page 612
Total pages 7
Editor Tony Flint
Place of publication Canberra, ACT, Australia
Publisher CSIRO Publishing
Collection year 2011
Language eng
Formatted abstract
The effect of supplementation of sex-sorted and non-sorted spermatozoa with seminal plasma protein (SPP) on fertility after cervical insemination was examined in the present study. Spermatozoa were sorted into high purity X and Y chromosome-bearing spermatozoa or not sorted and then either supplemented with SPP (>10 kDa) before freezing and/or after thawing (non-sorted only) or processed without supplementation. Inseminations were performed over 2 days with ewes receiving 100 or 25 million motile non-sorted spermatozoa in the cervix or uterus, respectively, or two cervical inseminations of 3.5 million motile sorted spermatozoa. Pregnancy rates in cervically inseminated ewes were unaffected by supplementation of sorted or non-sorted spermatozoa with SPP before freezing compared with no supplementation. The effect of post-thaw supplementation of non-sorted spermatozoa with SPP on pregnancy rates after cervical insemination varied with the day of insemination (P < 0.05); fertility was similar to laparoscopic insemination on Day 1 (56.0 ± 10.2% v. 58.6 ± 10.1%), but not on Day 2 (23.1 ± 7.4% v. 66.7 ± 9.2%). In conclusion, under the conditions of the present study, SPP did not consistently improve pregnancy rates after cervical insemination with frozen–thawed ram spermatozoa. This is the first report of pregnancies (5/56 ewes inseminated) after cervical insemination with frozen–thawed sex-sorted ram spermatozoa. Although the success rate is low, the findings are encouraging because ewes inseminated with the sex-sorted spermatozoa received only 7% of the recommended dose (100 million motile) for cervical insemination of frozen–thawed spermatozoa.
© CSIRO 2010
Keyword Cryopreservation
Pregnancy rate
Sex preselection
Sheep
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 04 Apr 2011, 14:09:59 EST by Dr Tamara Leahy on behalf of !NON-HERDC