Seasonal variation in the protective effect of seminal plasma on frozen-thawed ram spermatozoa

Leahy, T., Marti, J. I., Evans, G. and Maxwell, W. M. C. (2010) Seasonal variation in the protective effect of seminal plasma on frozen-thawed ram spermatozoa. Animal Reproduction Science, 119 1-2: 147-153. doi:10.1016/j.anireprosci.2009.12.010

Author Leahy, T.
Marti, J. I.
Evans, G.
Maxwell, W. M. C.
Title Seasonal variation in the protective effect of seminal plasma on frozen-thawed ram spermatozoa
Journal name Animal Reproduction Science   Check publisher's open access policy
ISSN 0378-4320
Publication date 2010-05
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.anireprosci.2009.12.010
Volume 119
Issue 1-2
Start page 147
End page 153
Total pages 7
Editor A. C. O. Evans
G. N. Hinch
J. E. Kinder
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
The response of ram spermatozoa to seminal plasma is highly variable, in part due to the presence of both stimulatory and inhibitory factors. The aim of this study was to assess variation in the protection of ram spermatozoa during freezing by seminal plasma. The seminal plasma variables studied were season of collection, fractionation and method of supplementation. Spermatozoa were supplemented before freezing with 4 mg of seminal plasma proteins (SPPs) per 108 cells, and their motility and viability were assessed during post-thaw incubation (37 °C). In Experiment 1, semen was (a) frozen with no supplementation (Control) (b) extended with a Tris-based diluent (C+TRIS), or (c) supplemented with seminal plasma collected throughout the year (in the Southern Hemisphere) and pooled for January–March, April–June, July–August and October–December, and either fractionated to produce a concentrated >10 kDa seminal plasma protein retentate (>10 kDa SPP), or kept as crude seminal plasma (CP). There was no effect of season or seminal plasma type (CP or >10 kDa SPP) on motility of spermatozoa. CP and >10 kDa SPP improved the viability of spermatozoa when collected from January–September compared to Control. Supplementation with >10 kDa SPP increased viability of spermatozoa, compared to CP, when collected from January to July. In Experiment 2, >10 kDa SPP were either added directly to the spermatozoa or included in the cryodiluent or >10 kDa SPP were not supplemented (Control). Both supplementation methods improved the motility and the proportion of viable, acrosome-intact spermatozoa but direct supplementation resulted in more viable, acrosome-intact spermatozoa compared with supplementation of the cryodiluent. These results show that supplementation of ram spermatozoa with CP, or its protein component (>10 kDa SPP), before freezing protects them from freeze–thaw damage. The protective effect is greatest when seminal plasma is collected during the breeding season, fractionated with >10 kDa filters and added directly to the spermatozoa.
© 2009 Elsevier B.V. All rights reserved.
Keyword Cryopreservation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Available online 16 December 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 04 Apr 2011, 14:08:07 EST by Dr Tamara Leahy on behalf of School of Veterinary Science