Seminal plasma proteins protect flow-sorted ram spermatozoa from freeze–thaw damage

Leahy, T., Marti, J. I., Evans, G. and Maxwell, W. M. C. (2009) Seminal plasma proteins protect flow-sorted ram spermatozoa from freeze–thaw damage. Reproduction Fertility and Development, 21 4: 571-578. doi:10.1071/RD08238

Author Leahy, T.
Marti, J. I.
Evans, G.
Maxwell, W. M. C.
Title Seminal plasma proteins protect flow-sorted ram spermatozoa from freeze–thaw damage
Journal name Reproduction Fertility and Development   Check publisher's open access policy
ISSN 1031-3613
Publication date 2009
Sub-type Article (original research)
DOI 10.1071/RD08238
Volume 21
Issue 4
Start page 571
End page 578
Total pages 8
Place of publication Canberra, ACT, Australia
Publisher C S I R O Publishing
Language eng
Formatted abstract
Seminal plasma improves the functional integrity of compromised ram spermatozoa but has been reported to be toxic to sorted spermatozoa. The present study attempted to clarify this paradoxical effect and improve the functional integrity of spermatozoa following sorting and cryopreservation. The in vitro function of sorted spermatozoa (motility characteristics and membrane integrity) was examined after supplementation with differing concentrations and protein fractions of seminal plasma at various stages of the sorting and freezing process. For all experiments, spermatozoa (two males, n ≤ four ejaculates per male) were processed through a high-speed flow cytometer before cryopreservation, thawing and incubation for 6 h (37°C). Supplementation of crude seminal plasma (CP), its low molecular weight fraction (LP; <10 kDa) or protein-rich fraction (SPP; >10 kDa), immediately before freezing improved the functional integrity of sorted spermatozoa compared with no supplementation (control), whereas supplementation after thawing had no effect for CP and LP. The protective effect of seminal plasma was not altered by increasing the amount of protein supplementation. No toxic effect of CP, SPP or LP was evident even when supplemented at high protein concentrations. It is concluded that seminal plasma protein, if added to ram spermatozoa after sorting and before freezing, can improve post-thaw sperm quality and consequently the efficiency of sorting. This effect is most likely related to protection of the spermatozoa during freeze-thawing. © CSIRO 2009.
Keyword Cryopreservation
Flow cytometry
Sex preselection
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 04 Apr 2011, 14:01:39 EST by Dr Tamara Leahy on behalf of School of Veterinary Science