Association of the severity of cortical damage with the occurrence of spontaneous seizures and hyperexcitability in an animal model of posttraumatic epilepsy

Kharatishvili, Irina and Pitkänena, Asla (2010) Association of the severity of cortical damage with the occurrence of spontaneous seizures and hyperexcitability in an animal model of posttraumatic epilepsy. Epilepsy Research, 90 1-2: 47-59. doi:10.1016/j.eplepsyres.2010.03.007


Author Kharatishvili, Irina
Pitkänena, Asla
Title Association of the severity of cortical damage with the occurrence of spontaneous seizures and hyperexcitability in an animal model of posttraumatic epilepsy
Journal name Epilepsy Research   Check publisher's open access policy
ISSN 0920-1211
1872-6844
Publication date 2010-06
Sub-type Article (original research)
DOI 10.1016/j.eplepsyres.2010.03.007
Volume 90
Issue 1-2
Start page 47
End page 59
Total pages 13
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
Posttraumatic epilepsy is a common consequence of traumatic brain injury in humans. Major predictors for the development of posttraumatic epilepsy include the severity of injury and occurrence of cortical contusions. The effect of the size or location of the cortical lesion on the risk of epileptogenesis, however, is poorly understood. Here, we investigated the extent and location of cortical damage and its association with a lowered seizure threshold and the occurrence of spontaneous seizures in rats (n = 77) that had experienced moderate or severe lateral fluid-percussion brain injury (FPBI) 12 months earlier. Spontaneous seizures were detected with video-electroencephalography monitoring and a lowered seizure threshold was determined based on a pentylenetetrazol (PTZ) test. Cortical atrophy was evaluated from thionin-stained sections using the Cavalieri estimation in four different experiments in which rats developed either spontaneous recurrent seizures (i.e., epilepsy) or a lowered seizure threshold. Our data show that damage to the cortex ipsilateral to the injury was more severe and extended more caudally in epileptic animals than in those without epilepsy (p < 0.05 and p < 0.001 for 2 independent experiments). Further, the extent of the cortical damage correlated positively with chronically increased hyperexcitability (number of spikes in PTZ test) in animals with traumatic brain injury (r = −0.54, p < 0.05; r = −0.72, p < 0.01 for 2 independent experiments). Specifically, cortical lesions located at the level of the perirhinal, entorhinal, and postrhinal cortices were associated with a lowered seizure threshold and seizures. The severity of the cortical injury did not correlate with the severity of hippocampal damage. These findings indicate that, like in humans, the severity of cortical injury correlates with epileptogenesis and epilepsy in an experimental model of posttraumatic epilepsy.
Copyright © 2010 Elsevier B.V. All rights reserved.
Keyword Cortical damage
Epilepsy
Fluid percussion brain injury
Histopathology
Hyperexcitability
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Centre for Advanced Imaging Publications
 
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Created: Mon, 28 Mar 2011, 16:16:45 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging